A Sialidase-Susceptible Ganglioside, IV3α(NeuGcα2-8NeuGc)-Gg4Cer, Is a Major Disialoganglioside in WHT/Ht Mouse Thymoma and Thymocytes
A Sialidase-Susceptible Ganglioside, IV3α(NeuGcα2-8NeuGc)-Gg4Cer, Is a Major Disialoganglioside in WHT/Ht Mouse Thymoma and Thymocytes
复制标题
对唾液酸酶敏感的神经节苷脂，IV3α(NeuGcα2-8NeuGc)-Gg4Cer，是 WHT/Ht 小鼠胸腺瘤和胸腺细胞中的主要二唾液酸神经节苷脂
DOI：
--
复制
发表时间：
1991
期刊：
影响因子：
--
通讯作者：
A. Suzuki
中科院分区：
文献类型：
--
作者：
Kyoko Nakamura;Minoru Suzuki;C. Taya;F. Inagaki;T. Yamakawa;A. Suzuki
对WHT/Ht小鼠胸腺瘤的双唾液酸神经节苷脂进行了纯化和表征，所述胸腺瘤是通过皮下移植在WHT/Ht小鼠中自发发展的胸腺瘤而获得的。根据糖组成分析、全甲基化研究、酶水解以及随后的TLC免疫染色、负离子快原子轰击质谱（FAB/MS）和1H-NMR光谱的结果，五种纯化的二唾液酸神经节苷脂之一的结构被确定为IV 3 α（NeuGc α 2- 8 NeuGc）-Gg 4Cer。基于唾液酸酶处理，随后用霍乱毒素B亚单位和抗Gg 4 Cer抗体进行TLC免疫染色，对其他4种二唾液酸神经节苷脂进行初步表征，结果为IV α（NeuAc α-NeuGc）-Gg 4 Cer、IV α（NeuGc α-NeuAc）-Gg 4 Cer、IV α NeuAc、II 3 α NeuAc-Gg 4 Cer和IV α NeuGc、II 3 α NeuGc-Gg 4 Cer。此外，在TLC上显示一个斑点的另一种组分是IV α NeuGc，II 3 α NeuAc-Gg 4Cer和IV α NeuAc，II 3 α NeuGc-Gg 4Cer的混合物。然后检查这些神经节苷脂在WHT/Ht小鼠胸腺细胞中的存在。作为两种主要的二唾液酸神经节苷脂之一，胸腺细胞含有IV 3 α（NeuGc α 2- 8 NeuGc）-Gg 4Cer，其特征在于通过微型高效液相色谱-FAB/MS获得的质谱和质谱图。另一种主要的二唾液酸神经节苷脂通过唾液酸酶处理，然后通过TLC-免疫染色初步表征为II 3 α-（NeuGc α-NeuGc）-Gg 4Cer。据报道，唾液酸酶敏感的单唾液酸神经节苷脂IV 3 α NeuGc-Gg 4 Cer [GM 1b（NeuGc）]是小鼠免疫组织的特征[中村，K.等人（1988）J. Biochem，103，201-208]。综上所述，结果表明，从Gg 4Cer到IV 3 α（NeuGc α 2- 8 NeuGc）-Gg 4Cer通过GM 1b（NeuGc）的途径在小鼠免疫组织中相当活跃。
The disialogangliosides of WHT/Ht mouse thymomas, which were obtained by subcutaneous transplantation of a thymoma that developed spontaneously in a WHT/Ht mouse, were purified and characterized. From the results of sugar-composition analysis, a permethylation study, enzymatic hydrolysis followed by TLC-immunostaining, negative-ion fast atom bombardment mass spectrometry (FAB/MS), and 1H-NMR spectroscopy, the structure of one of the five purified disialogangliosides was determined to be IV3 alpha(NeuGc alpha 2-8NeuGc)-Gg4Cer. The other 4 disialogangliosides were tentatively characterized on the basis of sialidase treatment followed by TLC-immunostaining with cholera toxin B subunit and anti-Gg4Cer antibody to be IV alpha(NeuAc alpha-NeuGc)-Gg4Cer, IV alpha(NeuGc alpha-NeuAc)-Gg4Cer, IV alpha NeuAc,II3 alpha NeuAc-Gg4Cer, and IV alpha NeuGc,II3 alpha NeuGc-Gg4Cer. In addition, another component exhibiting one spot on TLC was a mixture of IV alpha NeuGc,II3 alpha NeuAc-Gg4Cer and IV alpha NeuAc,II3 alpha NeuGc-Gg4Cer. Then the occurrence of these gangliosides in WHT/Ht mouse thymocytes was examined. As one of two major disialogangliosides, the thymocytes contained IV3 alpha(NeuGc alpha 2-8NeuGc)-Gg4Cer, which was characterized with a mass spectrum and mass chromatograms obtained by micro high-performance liquid chromatography-FAB/MS. The other major disialoganglioside was tentatively characterized to be II3 alpha-(NeuGc alpha-NeuGc)-Gg4Cer by sialidase treatment followed by TLC-immunostaining. A sialidase-susceptible monosialoganglioside, IV3 alpha NeuGc-Gg4Cer [GM1b(NeuGc)], had been reported to be characteristic of mouse immune tissues [Nakamura, K. et al. (1988) J. Biochem, 103, 201-208]. Taken together, the results suggest that the pathway from Gg4Cer to IV3 alpha(NeuGc alpha 2-8NeuGc)-Gg4Cer through GM1b(NeuGc) is quite active in mouse immune tissues.