Lung cancer lymph node micrometastasis detection using real-time polymerase chain reaction: correlation with vascular endothelial growth factor expression.
Lung cancer lymph node micrometastasis detection using real-time polymerase chain reaction: correlation with vascular endothelial growth factor expression.
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DOI：
10.1016/j.jtcvs.2012.12.023
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发表时间：
2013-03
影响因子：
6
通讯作者：
Demmy, Todd L.
中科院分区：
文献类型：
--
作者：
Nwogu, Chukwumere E.;Yendamuri, Sai;Tan, Wei;Kannisto, Eric;Bogner, Paul;Morrison, Carl;Cheney, Richard;Dexter, Elisabeth;Picone, Anthony;Hennon, Mark;Hutson, Alan;Reid, Mary;Adjei, Alex;Demmy, Todd L.
淋巴结（LN）分期提供了非小细胞肺癌（NSCLC）患者的关键信息。淋巴管生成可能是淋巴转移的病理生理学的重要贡献者。我们假设淋巴结微转移的存在与淋巴结中VEGF-A/C/D和VEGF受体-3（淋巴管生成因子）的表达呈正相关。40例NSCLC患者接受了术前PET-CT和纵隔镜检查。在选定的氟代脱氧葡萄糖（FDG）-亲合淋巴结中进行上皮标记物（CK-7，CEACAM-5和PLODN）的mRNA表达的RT-PCR测定。VEGF-A/C/D和VEGF-受体-3的表达水平在原发肿瘤和淋巴结中进行了测量。对LN中RT-PCR上皮标记物水平和VEGF表达水平之间的关联进行Wilcoxon秩和检验。CK-7、CEACAM 5或PLG的RT-PCR显示35例患者中有19例（54%）存在淋巴结微转移。微转移的检测与淋巴结中VEGF-A/C/D或VEGF-receptor-3的表达水平高度相关。中位随访时间为12.6个月。FDG亲合淋巴结的RT-PCR分析导致患者的分期升高。NSCLC患者淋巴结微转移与VEGF表达相关。这可能反映了淋巴管生成在促进转移中的作用。
Lymph node (LN) staging provides critical information in non-small cell lung cancer (NSCLC) patients. Lymphangiogenesis may be an important contributor to the pathophysiology of lymphatic metastases. We hypothesized that the presence of lymph node micrometastases positively correlates with VEGF-A/C/D and VEGF-receptor-3 (lymphangiogenic factors) expression in lymph nodes. Forty NSCLC patients had pre-operative PET-CT and mediastinoscopy. RT-PCR assays for mRNA expression of epithelial markers (CK-7, CEACAM-5 and PLUNC) were performed in selected fluorodeoxyglucose (FDG)-avid lymph nodes. VEGF-A/C/D and VEGF-receptor-3 expression levels were measured in primary tumors and lymph nodes. Wilcoxon rank sum test was run for the association between the RT-PCR epithelial marker levels and VEGF expression levels in the LNs. RT-PCR for CK-7, CEACAM5 or PLUNC indicated lymph node micrometastatic disease in 19 of 35 patients (54%). There was a high correlation between detection of micrometastases and VEGF-A/C/D or VEGF-receptor-3 expression levels in lymph nodes. Median follow-up was 12.6 months. RT-PCR analysis of FDG-avid lymph nodes results in up-staging of patients. Micrometastases correlate with the expression of VEGF in lymph nodes in NSCLC patients. This may reflect the role of lymphangiogenesis in promoting metastases.
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DOI：
10.1196/annals.1413.020
发表时间：
2008-01-01
期刊：
LYMPHATIC CONTINUUM REVISITED
影响因子：
--
作者：
Achen, Marc G.;Stacker, Steven A.
通讯作者：
Stacker, Steven A.
影响因子：
20.4
作者：
Goldstraw, Peter;Crowley, John;Sobin, Leslie
通讯作者：
Sobin, Leslie
影响因子：
9.6
作者：
Mountain, CF;Dresler, CM
通讯作者：
Dresler, CM
影响因子：
4.6
作者：
Nwogu, Chumy;Fischer, Gabor;Demmy, Todd
通讯作者：
Demmy, Todd
影响因子：
6.2
作者：
Pulte, D;Li, E;Jacobson, DR
通讯作者：
Jacobson, DR