Excitation wavelength-dependent changes in Raman spectra of whole blood and hemoglobin: comparison of the spectra with 514.5-, 720-, and 1064-nm excitation
Excitation wavelength-dependent changes in Raman spectra of whole blood and hemoglobin: comparison of the spectra with 514.5-, 720-, and 1064-nm excitation
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DOI：
10.1117/1.1380668
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发表时间：
2001-07-01
影响因子：
3.5
通讯作者：
Ozaki, Y
中科院分区：
文献类型：
--
作者：
Sato, H;Chiba, H;Ozaki, Y
在相同的条件下，用可见光（514.5 nm）和近红外（NIR; 720和1064 nm）激发，测量全血和氧合血红蛋白（Hb）的拉曼光谱，并详细比较所获得的光谱。用可见光激发的血液的拉曼光谱由类胡萝卜素引起的非常强的带主导，使得难以从光谱获得关于Hb的信息。用720 nm光激发的全血和氧合血红蛋白的拉曼光谱彼此非常接近;这两个光谱基本上是与Q带预共振的血红素发色团的拉曼光谱。对720 nm激发下的拉曼光谱进行了定性分析，包括谱带归属和共振效应的性质研究。用1064 nm光激发的全血和氧-Hb的光谱包含来自血红素发色团的非共振拉曼光谱和蛋白质的拉曼光谱的贡献。血液和氧合血红蛋白的1064 nm激发光谱彼此相似，但在某些特征上有所不同。例如，在全血的光谱中，蛋白质的谱带比氧合血红蛋白的谱带更强，氧合血红蛋白除了珠蛋白部分外不含蛋白质。通过对514.5、720和1064 nm激发拉曼光谱的比较，发现720 nm的激发波长比可见光和1064 nm的激发波长更适用于血红蛋白的拉曼分析，如氧合分析，特别是原位测量。(C)2001年，美国光电仪器工程师学会。
Raman spectra of whole blood and oxy-hemoglobin (Hb) were measured under the same conditions with visible (514.5 nm) and near-infrared (NIR; 720 and 1064 nm) excitation, and the obtained spectra were compared in detail. The Raman spectrum of blood excited with visible light is dominated by very intense bands due to carotenoids, so that it was difficult to obtain information about Hb from the spectrum. The Raman spectra of whole blood and oxy-Hb excited with 720 nm light are very close to each other; both spectra are essentially Raman spectra of the heme chromophore that is preresonant with Q bands. Qualitative spectral analysis including band assignment and investigation of nature of resonance effect were carried out for the Raman spectra with 720 nm excitation. The spectra of whole blood and oxy-Hb excited with 1064 nm light contain contributions from nonresonance Raman spectra of the heme chromophore and Raman spectra of proteins. The 1064 nm excited spectra of blood and oxy-Hb are similar to each other but different in some features. For example, bands due to protein appear stronger in the spectrum of whole blood than in that of oxy-Hb which does not contain protein except globin part. The comparison between the 514.5, 720, and 1064 nm excited Raman spectra reveal that the excitation wavelength of 720 nm is more practical than that of visible light and 1064 nm in the Raman analysis of Hb, such as oxygenation, specially in situ measurement. (C) 2001 Society of Photo-Optical Instrumentation Engineers.