Stimulation of encephalomyocarditis virus RNA translation in wheat embryo extracts by rabbit reticulocyte ribosomal wash.
Stimulation of encephalomyocarditis virus RNA translation in wheat embryo extracts by rabbit reticulocyte ribosomal wash.
复制标题
兔网织红细胞核糖体洗涤刺激小麦胚胎提取物中脑心肌炎病毒 RNA 翻译。
DOI：
10.1128/jvi.35.2.566-571.1980
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发表时间：
1980
影响因子：
5.4
通讯作者：
Kaesberg,P
中科院分区：
文献类型：
--
作者：
Shih,DS;Shih,CY;Kaesberg,P
脑心肌炎病毒RNA诱导小麦胚提取物中低水平的氨基酸掺入，但掺入不导致任何病毒蛋白的形成。然而，如果在小麦胚提取物中添加兔网织红细胞核糖体洗涤部分，则可以获得大量氨基酸掺入和脑心肌炎病毒特异性蛋白质形成的刺激。合成的蛋白中有衣壳前体蛋白，可被脑心肌炎病毒编码的蛋白酶加工成病毒衣壳蛋白。
Encephalomyocarditis virus RNA induced a low level of amino acid incorporation in wheat embryo extracts, but the incorporation did not result in the formation of any viral proteins. However, if wheat embryo extracts were supplemented with a ribosomal wash fraction from rabbit reticulocytes, a large stimulation of amino acid incorporation and the formation of encephalomyocarditis virus-specific proteins were obtained. Among the proteins synthesized were capsid precursor proteins which can be processed by an encephalomyocarditis virus-coded protease to produce the viral capsid proteins.