Multiplexed Resistive Pulse Sensor Based on Geometry Modulation for High-throughput Microparticle Counting
Multiplexed Resistive Pulse Sensor Based on Geometry Modulation for High-throughput Microparticle Counting
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DOI：
10.1016/j.snr.2023.100140
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发表时间：
2023-01
影响因子：
5.9
通讯作者：
Ruiting Xu;Leixin Ouyang;Rubia Shaik;Ge Zhang;J. Zhe
中科院分区：
文献类型：
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作者：
Ruiting Xu;Leixin Ouyang;Rubia Shaik;Ge Zhang;J. Zhe
而阻性脉冲传感器(RPS)已被用来表征纳米/微米靶标(细胞、生物分子等)。在生物医学研究中，一个长期存在的缺点是其吞吐量低。在这里，我们报告了一种新的基于几何调制的RPS，在不增加测量电子学复杂性的情况下提高了吞吐量。该传感器由多个并行传感通道组成，其几何结构基于7比特扩频序列进行了独特的设计。由于独特的几何形状，当粒子通过传感通道时，来自该通道的电压信号由特定的波形编码。只施加了一个直流源，并且只采集了来自所有传感通道的一个组合信号。对于解调，使用组合信号和每个模板波形之间的最大相关系数来识别来自特定传感通道的粒子的通过，以及该通过的发生时间。提出了一种迭代抵消方案，通过对识别的波形进行从高到低的一系列减法，直到剩余信号与所有模板波形之间的相关系数小于0.4(弱相关性)为止。不同大小的聚苯乙烯颗粒的混合物被用来测试该设备。结果表明，该装置能够准确测量和计数各种微粒，测量误差分别为5.8%和5.2%，处理量提高了300%。几何调制RPS结构简单，测量装置简单，在各种微纳米生物物体的检测和分析中具有很大的潜力。
While resistive pulse sensor (RPS) has been used to characterize the nano/micro-targets (cells, biomolecules, etc.) in biomedical research, one long standing drawback is its low throughput. Here we report a novel geometry modulation based RPS to improve the throughput without increasing the complexity of measurement electronics. The sensor consists of multiple parallel sensing channels whose geometries are uniquely designed based on 7-bit spreading sequences. Because of the unique geometry, when a particle passes a sensing channel, the voltage signal from this channel is encoded by a specific waveform. Only a DC source was applied, and only one combined signal from all sensing channels was collected. For demodulation, the maximum correlation coefficient between the combined signal and each template waveform was used to identify the passage of a particle from a specific sensing channel, and the occurring time of the passage. An iterative cancellation scheme was developed to extract the identified waveforms, by a series of subtractions of the identified waveforms with amplitudes from high to low, until the correlation coefficients between the remaining signal with all template waveforms became less than 0.4 (weak correlation). Mixtures of different-sized polystyrene particles were used to test the device. Results showed that the device is capable of accurately sizing and counting various microparticles with errors of 5.8% and 5.2% while the throughput was improved 300%. With the simple structure and measurement setup, the geometry-modulated RPS has great potential for the detection and analysis of a variety of micro/nano bio-objects.