“Plug-and-Play Microfluidic Production of Monodisperse Giant Unilamellar Vesicles Using Droplet Transfer across Water-Oil Interface”
“Plug-and-Play Microfluidic Production of Monodisperse Giant Unilamellar Vesicles Using Droplet Transfer across Water-Oil Interface”
复制标题
“利用水-油界面上的液滴传输即插即用微流体生产单分散巨型单层囊泡”
DOI：
10.1016/j.snb.2021.131281
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
H. Suzuki
中科院分区：
文献类型：
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作者：
R. Ushiyama;K. Koiwai;H. Suzuki
巨型单层囊泡（GUVs）是一种细胞大小的囊室，广泛应用于生物和化学领域。在仿生微反应器研究中，guv的单分散性对定量分析和实验重现性至关重要。在这项研究中，我们建立了一种高重复性的即插即用微流体方法来产生单分散的guv，而无需对通道进行表面处理或精确的流量调节。在微流体通道中，我们通过流体动力聚焦产生油包水（W/O）液滴，并通过W - O界面转移形成薄壳油包水（W/O/W）液滴，作为guv的前体。通过稳定油水共流和调节W - O界面曲率，W/O液滴的传递成功率接近100%。由于界面能最小化，W/O/W液滴的薄油层脱水并分离形成guv。我们进行了膜蛋白插入试验，以证实guv的单侧性及其由渗透作用引起的收缩。
Giant unilamellar vesicles (GUVs) are cell-sized compartments widely used in biological and chemical applications. The monodispersity of GUVs is essential for quantitative analysis and experimental reproducibility in biomimetic microreactor studies. In this study, we established a highly reproducible plug-and-play microfluidics-based method to generate monodisperse GUVs without surface treatment of the channel or precise flow-rate adjustment. In the microfluidic channel, we generated water-in-oil (W/O) droplets through hydrodynamic flow focusing and transferred across the W–O interface to form thin-shelled water-in-oil-in-water (W/O/W) droplets as precursors to GUVs. The success rate of the W/O droplet transfer reached nearly 100% by stabilizing the co-flow of oil and water and adjusting the curvature of the W–O interface. Owing to interfacial energy minimization, the thin oil layer of the W/O/W droplets dewetted and detached to form GUVs. We performed a membrane-protein insertion assay to confirm the unilamellarity of the GUVs and their shrinkage caused by osmotic action.