In vitro and in vivo anti-tumor effects of Astragalus membranaceus
In vitro and in vivo anti-tumor effects of Astragalus membranaceus
复制标题
DOI：
10.1016/j.canlet.2006.12.001
复制
发表时间：
2007-07-08
期刊：
影响因子：
9.7
通讯作者：
Leung, Kwok N.
中科院分区：
文献类型：
--
作者：
Cho, William C. S.;Leung, Kwok N.
黄芪是一种常用的中药植物，已被证明能够恢复癌症患者受损的T细胞功能。本研究观察了A.膜壳菌的研究。从黄芪的根中分离得到5个活性部位。在膜壳菌中，被指定为AI的级分被发现在五个级分中就其对鼠脾细胞的促有丝分裂性而言是最有效的。除了研究AI的细胞抑制作用外，还研究了其对巨噬细胞功能、肿瘤坏死因子产生、淋巴因子激活的杀伤细胞诱导和肿瘤细胞分化的活性。巨噬细胞样肿瘤和髓系肿瘤对AI的细胞抑制活性更敏感，而成纤维细胞样肿瘤和小鼠埃利希腹水瘤似乎相对抵抗。AI能有效抑制小鼠体内同系肿瘤的生长。结果表明，经AI预处理的小鼠巨噬细胞对MBL-2肿瘤的体外和体内抑制活性均增强。AI还可作为荷瘤小鼠肿瘤坏死因子产生的引发剂。AI预孵育小鼠脾细胞可诱导淋巴因子激活的对WEHI-164细胞的杀伤活性。此外，AI能够在体外诱导人和小鼠细胞的单核细胞分化。体内给药AI甚至可以部分恢复荷瘤小鼠中抑制的促有丝分裂反应。结果表明，A.黄芪在体内外均具有抗肿瘤作用，其抗肿瘤作用可能是通过激活宿主的抗肿瘤免疫机制实现的。(c)2006爱思唯尔爱尔兰有限公司保留所有权利。
Astragalus membranaceus, a commonly used Chinese medicinal plant, has been shown to be capable of restoring the impaired T cell functions in cancer patients. In this study, the in vitro and in vivo anti-tumor effects of A. membranaceus were investigated. Five bioactive fractions were isolated from the root of A. membranaceus, the fraction designated as AI was found to be the most potent among the five fractions with respect to its mitogenicity on murine splenocytes. Besides investigating the cytostatic effect of AI, its activities on macrophage function, tumor necrosis factor production, induction of lymphokine-activated killer cell and tumor cell differentiation were also examined. The macrophage-like tumors and the myeloid tumors were found to be more sensitive to the cytostatic activity of AI, whereas the fibroblast-like tumors and the mouse Ehrlich ascites tumor appeared to be relatively resistant. Moreover, AI could effectively suppress the in vivo growth of syngeneic tumor in mice. Results showed that murine macrophage pretreated with AI had increased in vitro and in vivo cytostatic activities towards MBL-2 tumor. AI could also act as a priming agent for tumor necrosis factor production in tumor-bearing mice. Preincubation of mouse splenocytes with AI could induce in vitro lymphokine-activated killer-like activity towards WEHI-164 cell. Furthermore, AI was able to induce monocytic differentiation of both human and murine cells in vitro. AI administered in vivo could even partially restore the depressed mitogenic response in tumor-bearing mice. Collectively, the results showed that A. membranaceus could exhibit both in vitro and in vivo anti-tumor effects, which might be achieved through activating the anti-tumor immune mechanism of the host. (c) 2006 Elsevier Ireland Ltd. All rights reserved.