Matrix metalloproteinase-9 expression in the normal mucosa-adenoma-dysplasia-adenocarcinoma sequence of the colon
Matrix metalloproteinase-9 expression in the normal mucosa-adenoma-dysplasia-adenocarcinoma sequence of the colon
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DOI：
10.1007/s12253-008-9004-5
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发表时间：
2008-03-01
影响因子：
2.8
通讯作者：
Tulassay, Zsolt
中科院分区：
文献类型：
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作者：
Herszenyi, Laszlo;Sipos, Ferenc;Tulassay, Zsolt
已有研究表明，基质金属蛋白酶(MMPs)在肿瘤侵袭中起重要作用。我们检测了基质金属蛋白酶-9(MMP9)在结直肠癌、相应的正常粘膜和结直肠腺瘤中的蛋白表达。为确认免疫组织化学结果，进行了基质金属蛋白酶-9 TaqMan RT-PCR分析。应用免疫组织化学方法检测31例大肠癌患者(癌组织及相应的正常粘膜)和30例腺瘤(9例腺瘤伴高度不典型增生)石蜡包埋活检切片中MMP9的表达。半定量检测基质金属蛋白酶-9免疫组化染色。Taqman RT-PCR检测正常粘膜5例，腺瘤不典型增生6例，高度不典型增生7例，结直肠癌10例。统计分析采用单因素方差分析、LSD检验和相关分析。的P值
It has been proposed that matrix metalloproteinases (MMPs) play a role in tumor invasion. We determined protein expression of matrix metalloproteinase-9 (MMP-9) in colorectal cancer (CRC), corresponding normal mucosa and colorectal adenomas. For confirmation of immunohistochemical results MMP-9 TaqMan RT-PCR analysis was performed. Expression of MMP-9 was determined on paraffin embedded biopsy sections by immunohistochemistry in 31 CRC patients (from cancer tissue and corresponding normal mucosa) and in 30 patients with adenoma (nine adenomas with high grade of dysplasia). MMP-9 immunostaining was determined semi-quantitatively. For Taqman RT-PCR analyses normal mucosa (n=5), adenoma without (n=6) and with high grade dysplasia (n=7) and CRC (n=10) were investigated. Statistical analysis with ANOVA, LSD test and correlation analysis were performed. P value of