Architecture of recombination intermediates visualized by in-gel FRET of lambda integrase-Holliday junction-arm DNA complexes.
Architecture of recombination intermediates visualized by in-gel FRET of lambda integrase-Holliday junction-arm DNA complexes.
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通过 lambda 整合酶-Holliday 连接臂 DNA 复合物的凝胶内 FRET 可视化重组中间体的结构。
DOI：
10.1073/pnas.0500844102
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Landy,Arthur
中科院分区：
文献类型：
--
作者：
Radman-Livaja,Marta;Biswas,Tapan;Mierke,Dale;Landy,Arthur
λ Integrase (Int) mediates recombination between attachment sites on phage andEscherichia coliDNA. Int is assisted by accessory protein-induced DNA loops in bridging pairs of distinct “arm-type” and “core-type” DNA sites to form synapsed recombination complexes that subsequently recombine by means of a Holliday junction (HJ) intermediate. An in-gel FRET assay was developed and used to measure 15 distances between six points in two Int–HJ complexes containing arm-DNA oligonucleotides, and 3D maps of these complexes were derived by distance-geometry calculations. The maps reveal unexpected positions for the arm-type DNAs relative to core sites on the HJ and a new Int conformation in the HJ tetramer. The results show how the position of arm DNAs determines the bias of catalytic activities responsible for directional resolution, provide insights into the organization of Int higher-order complexes, and lead to models of the structure of the full HJ recombination intermediates.
