Urine cell-free microRNA as biomarkers for transitional cell carcinoma.
Urine cell-free microRNA as biomarkers for transitional cell carcinoma.
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DOI：
10.1186/s13104-017-2950-9
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发表时间：
2017-11-29
影响因子：
1.8
通讯作者：
Ben-Dov IZ
中科院分区：
文献类型：
--
作者：
Geva GA;Gielchinsky I;Aviv N;Max KEA;Gofrit ON;Gur-Wahnon D;Ben-Dov IZ
MicroRNA（miRNA）是一种在细胞中具有调节功能的短核苷酸链。几种miRNA已被证明可用作不同肿瘤的生物标志物。该项目的目的是评估无细胞尿中的miRNA水平是否可用作移行细胞癌（TCC）的生物标志物。根据14名TCC患者和20名健康志愿者的尿液样品中的小RNA产生cDNA文库。在Illumina HiSeq测序仪上对所得读数进行深度测序，目的是表征无细胞尿miRNA谱。发现单个miRNA存在统计学显著差异;与对照组相比，TCC组中的miR-210高出6倍以上。此外，我们能够通过对过表达的miRNA的标准化水平求和来产生诊断评分。该评分在TCC患者中相当高，敏感性为0.93，特异性为0.76，阴性预测值> 0.97。本文的在线版本（10.1186/s13104-017-2950-9）包含补充材料，可供授权用户使用。
MicroRNA (miRNA) are short nucleotide strands with a regulatory function in the cell. Several miRNAs have been shown to be useful as biomarkers for different neoplasms. The aim of this project was to assess whether levels of miRNA in cell free urine could be used as a biomarker in transitional cell carcinoma (TCC). cDNA libraries were produced based on small RNAs in urine samples of fourteen TCC patients and twenty healthy volunteers. Resulting reads were deep sequenced on Illumina HiSeq sequencer with the intent of characterizing cell free urine miRNA profiles. A statistically significant difference was found for a single miRNA; miR-210 was > sixfold higher in the TCC group compared to the control group. Furthermore, we were able to produce a diagnostic score by summing of standardized levels of overexpressed miRNA. This score was considerably higher in TCC patients with a sensitivity of 0.93, specificity of 0.76 and negative predictive value > 0.97. The online version of this article (10.1186/s13104-017-2950-9) contains supplementary material, which is available to authorized users.