FERONIA receptor-like kinase regulates RHO GTPase signaling of root hair development
FERONIA receptor-like kinase regulates RHO GTPase signaling of root hair development
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DOI：
10.1073/pnas.1005366107
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发表时间：
2010-10-12
影响因子：
11.1
通讯作者：
Wu, Hen-Ming
中科院分区：
文献类型：
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作者：
Duan, Qiaohong;Kita, Daniel;Wu, Hen-Ming
植物Rho GTPases（RAC/ROPS）介导了从激素到压力的多个细胞外信号，并调节对极化细胞生长，分化，发育，繁殖和对环境反应的多种细胞过程。他们在GDP结合的非活性状态与GTP结合的活化状态之间穿梭，其激活主要由称为RopGefs的鸟嘌呤核苷酸交换因子（GEFS）介导。我们将拟南芥ROPGEF1作为诱饵，我们将受体样激酶（RLK）家族的成员确定为RAC/ROP信号传导的潜在上游调节剂。 NADPH氧化酶衍生的活性氧（ROS）成为生长和发育的重要调节剂，并在介导RAC/ROP调节的根部头发发育（一种极化的细胞生长过程）中起着至关重要的作用。因此，我们筛选了这些RLK中的T-DNA插入突变体，以使根毛缺损，发现其中一个突变是编码Feronia（FER）受体的AT3G51550，像激酶一样诱导了严重的根毛缺损。我们表明，FER表型与降低的活性RAC/ROP/ROPS和NADPH氧化酶依赖性，生长素调节的ROS在根和根毛中的积累以及FER中的RAC/ROP信号上调了突变体表型。综上所述，这些观察结果强烈支持FER作为RAC/ROP信号途径的上游调节剂，该途径控制ROS介导的根毛发育。此外，将FER通过ROP2 GTPase以鸟嘌呤核苷酸调节的方式拉下，这意味着涉及FER，ROPGEF和RAC/ROP的动态信号传导复合物。
Plant RHO GTPases (RAC/ROPs) mediate multiple extracellular signals ranging from hormone to stress and regulate diverse cellular processes important for polarized cell growth, differentiation, development, reproduction, and responses to the environment. They shuttle between the GDP-bound inactive state and the GTP-bound activated state and their activation is predominantly mediated by a family of guanine nucleotide exchange factors (GEFs) referred to as ROPGEFs. Using the Arabidopsis ROPGEF1 as bait, we identified members of a receptor-like kinase (RLK) family as potential upstream regulators for RAC/ROP signaling. NADPH oxidase-derived reactive oxygen species (ROS) are emerging as important regulators for growth and development and play a crucial role in mediating RAC/ROP-regulated root hair development, a polarized cell growth process. We therefore screened T-DNA insertion mutants in these RLKs for root hair defects and found that mutations in one of them, At3g51550 encoding the FERONIA (FER) receptor- like kinase, induced severe root hair defects. We show that the fer phenotypes correlated with reduced levels of active RAC/ROPs and NADPH oxidase-dependent, auxin-regulated ROS accumulation in roots and root hairs and that up-regulating RAC/ROP signaling in fer countered the mutant phenotypes. Taken together, these observations strongly support FER as an upstream regulator for the RAC/ROP-signaled pathway that controls ROS-mediated root hair development. Moreover, FER was pulled down by ROP2 GTPase in a guanine nucleotide-regulated manner implying a dynamic signaling complex involving FER, a ROPGEF, and a RAC/ROP.