Exo-miRExplorer: A Comprehensive Resource for Exploring and Comparatively Analyzing Exogenous MicroRNAs.
Exo-miRExplorer: A Comprehensive Resource for Exploring and Comparatively Analyzing Exogenous MicroRNAs.
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Exo-miRExplorer：探索和比较分析外源 MicroRNA 的综合资源
DOI：
10.3389/fmicb.2017.00126
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发表时间：
2017
影响因子：
5.2
通讯作者：
Qu LH
中科院分区：
文献类型：
--
作者：
Zheng LL;Deng KW;Deng AC;Wu J;Yang JH;Lun ZR;Qu LH
MicroRNA (miRNA) 是小调控 RNA，在动物、植物和病毒中发挥重要作用。深度测序技术已广泛应用于miRNA研究中。然而，为什么几乎所有测序数据都包含来自外源物种的 miRNA 序列（称为 exo-miRNA），这仍然是一个很大的谜团。在这项研究中，我们开发了一个新的平台 exo-miRExplorer，用于从源自多种生物体的组织和细胞系的高通量小 RNA 测序实验中挖掘和识别 exo-miRNA。 exo-miRExplorer 中提供了数千种 exo-miRNA 的表达丰度、RNA 家族、原始生物体和测序平台。随后，我们使用 exo-miRExplorer 进行进一步分析。对不同测序数据集之间的 exo-miRNA 的比较分析揭示了同一研究中实验之间的 exo-miRNA 的显着相关性。植物来源的 exo-miRNA 分析为 exo-miRNA 的非饮食来源提供了有力的证据。病毒来源的exo-miRNA分析表明，病原体RNA可以转移至宿主细胞，并以丰度水平存在于深度测序结果中。总之，exo-miRExplorer 为用户提供了一个综合资源，以促进 exo-miRNA 的检测和分析。 exo-miRExplorer 可通过以下 URL 获取：http://rna.sysu.edu.cn/exomiRDB/。
MicroRNAs (miRNAs) are small regulatory RNAs that play important roles in animals, plants, and viruses. Deep-sequencing technology has been widely adopted in miRNA investigations. However, it is still a big mysterious why nearly all sequencing data contain miRNA sequences from exogenous species, called exo-miRNAs. In this study, we developed a novel platform, exo-miRExplorer, for mining and identifying exo-miRNAs from high-throughput small RNA sequencing experiments which originated from tissues and cell lines of multiple organisms. Thousands of exo-miRNAs are characterized with their expression abundance, the RNA families, original organisms and the sequencing platforms presented in exo-miRExplorer. Subsequently, we used exo-miRExplorer to perform further analysis. Comparative analysis of the exo-miRNAs between different sequencing datasets revealed significant correlation of exo-miRNAs between experiments in the same study. The plant-derived exo-miRNAs analysis provided robust evidence for non-diet source of exo-miRNAs. Virus-derived exo-miRNA analysis showed that pathogen RNAs could transfer to host cells and exist in deep-sequencing result at abundance level. In conclusion, exo-miRExplorer provides users with an integrative resource to facilitate detection and analysis of exo-miRNAs. exo-miRExplorer is available at the following URL: http://rna.sysu.edu.cn/exomiRDB/.