Comprehensive circular RNA profiles in plasma reveals that circular RNAs can be used as novel biomarkers for systemic lupus erythematosus
Comprehensive circular RNA profiles in plasma reveals that circular RNAs can be used as novel biomarkers for systemic lupus erythematosus
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DOI：
10.1016/j.cca.2018.01.026
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发表时间：
2018-05-01
影响因子：
5
通讯作者：
Qiu, Yurong
中科院分区：
文献类型：
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作者：
Li, Haixia;Li, Kaifei;Qiu, Yurong
环状RNA（circular RNA，circRNA）是一类广泛存在的内源性非编码RNA，与动脉粥样硬化、阿尔茨海默病和多种癌症等多种疾病的发生发展密切相关，但其与系统性红斑狼疮（systemic lupus erythematosus，SLE）的关系尚不清楚。本研究旨在利用circRNA微阵列技术检测6对SLE患者和正常人血浆中circRNA的表达谱。微阵列分析显示，207个circRNA在这两个组之间差异表达，包括113个上调和94个下调circRNA。然后，我们从微阵列分析中选择8个circRNA作为候选生物标志物，并使用定量实时聚合酶链反应（qRT-PCR）在另一组由24名SLE患者和24名正常对照组成的受试者中进一步验证它们。最后，我们确认了四个与微阵列结果一致的circRNA。此外，生物信息学被用来预测验证的circRNA和潜在的miRNA靶之间的相互作用。综上所述，我们首次阐明了circRNA在SLE患者血浆中的全面表达谱，为将来开发circRNA作为SLE疾病的新型非侵入性生物标志物奠定了基础。
Circular RNAs (circRNAs), a novel class of widespread endogenous noncoding RNAs, have been involved in the development of various diseases, including atherosclerosis, Alzheimer's disease and several types of cancers, but there is little knowledge about their associations with systemic lupus erythematosus (SLE). This study is aimed to identify the expression profiles of circRNAs in 6 paired SLE and normal participants plasma samples by using a circRNA microarray. The microarray analysis showed that 207 circRNAs were differentially expressed between these two groups, including 113 upregulated and 94 downregulated circRNAs. Then, we selected 8 circRNAs as candidate biomarkers from the microarray analysis and further verified them in another group of subjects consisting of 24 SLE patients and 24 normal controls using quantitative real-time polymerase chain reaction assays (qRT-PCR). Finally, we confirmed four circRNAs that were consistent with the microarray results. In addition, bioinformatics was employed to predict the interaction between validated circRNAs and potential miRNA targets. Taken together, we firstly illustrate the comprehensive expression profiles of circRNAs in SLE patients plasma and lay the foundations to develop circRNAs as novel non-invasive biomarkers for SLE disease in the future.