Photosynthetic responses of thalli and isolated protoplasts of Bryopsis hypnoides (Bryopsidales, Chlorophyta) during dehydration
Photosynthetic responses of thalli and isolated protoplasts of Bryopsis hypnoides (Bryopsidales, Chlorophyta) during dehydration
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脱水过程中苔藓植物菌体和分离原生质体的光合反应
DOI：
10.1007/s00343-011-0028-4
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发表时间：
2011-03
影响因子：
--
通讯作者：
Jin Haochen
中科院分区：
文献类型：
--
作者：
Lue Fang;Lue Fang;Wang Guangce;Wang Guangce;Jin Haochen;Jin Haochen
催眠苔藓藻（Bryopsis hypnoides Lamouroux）是一种独特的潮间带管形绿色藻类，其挤出的原生质在海水中能自发聚集形成大量新细胞，并能发育成成熟的藻体。本文研究了从B中分离的菌体和原生质体在脱水过程中的光合反应。使用Dual-PAM（脉冲幅度调制）-100荧光计测量催眠药。结果表明，B. hypnoidesthalli维持了一个初始阶段，超过此持续干燥导致PSI和PSII的速率降低。而在空气（CO_2浓度600-700 mg/L）中脱水的原生质体，在失水20%时，Y（II）略有增加，但随着含水量的降低，Y（II）的光合速率下降。当原生质体在CO_2缺乏条件下脱水时（CO_2浓度为40-80 mg/L），Y（II）值随脱水程度的增加而稳定下降，而没有初始上升。这些结果表明，该菌的菌体和原生质体能够有效利用环境空气中的CO2，并且在脱水过程中，原生质体的光合性能与菌体有显著差异。因此，原生质体可能是研究植物抗逆性的一个很好的系统。
Bryopsis hypnoides Lamouroux is a unique intertidal siphonous green alga whose extruded protoplasm can aggregate spontaneously in seawater to form numerous new cells that can develop into mature algal thalli. In this study, the photosynthetic responses during dehydration of both the thalli and protoplasts isolated from B. hypnoides were measured using a Dual-PAM (pulse amplitude modulation)-100 fluorometer. The results show that the photosynthetic rates of B. hypnoides thalli were maintained for an initial period, beyond which continued desiccation resulted in reduced rates of PSI and PSII. However, the photosynthetic performances of the isolated protoplasts dehydrated in air (CO2 concentration 600–700 mg/L) showed a slight increase of Y(II) at 20% water loss, but the rates decreased thereafter with declining water content. When protoplasts were dehydrated in CO2 deficient conditions (CO2 concentration 40–80 mg/L), the values of Y(II) declined steadily with increased dehydration without an initial rise. These results indicated that the thalli and isolated protoplasts of this alga can utilize CO2 in ambient air effectively, and the photosynthetic performances of the isolated protoplasts were significantly different from that of the thalli during dehydration. Thus the protoplasts may be an excellent system for the study of stress tolerance.
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影响因子：
2.9
作者：
TATEWAKI M;NAGATA K
通讯作者：
NAGATA K
影响因子：
2.4
作者：
Markus Andersson;Hendrik Schubert;Marianne Pedersén;P. Snoeijs
通讯作者：
Markus Andersson;Hendrik Schubert;Marianne Pedersén;P. Snoeijs
影响因子：
2.5
作者：
J. Stapel;R. Manuntun;M. Hemminga
通讯作者：
J. Stapel;R. Manuntun;M. Hemminga
影响因子：
2.4
作者：
D. Zou;K. Gao
通讯作者：
D. Zou;K. Gao
影响因子：
4.8
作者：
JOHNSON, WS;GIGON, A;MOONEY, HA
通讯作者：
MOONEY, HA