Expression of mRNA for functional molecules in urinary sediment in glomerulonephritis
Expression of mRNA for functional molecules in urinary sediment in glomerulonephritis
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DOI：
10.1007/s00467-007-0683-z
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发表时间：
2008-03-01
影响因子：
3
通讯作者：
Tanaka, Hiroshi
中科院分区：
文献类型：
--
作者：
Tsugawa, Koji;Oki, Eishin;Tanaka, Hiroshi
最近的研究表明，利用尿沉积物进行基因表达研究可能是评估肾小球肾炎活性和发病机制的一种非侵入性方法。然而，关于免疫复合物介导的肾小球肾炎患者尿沉积物中功能分子（如T-bet、GATA-3、FOXP3和视黄酸诱导基因i (RIG-I)） mRNA表达模式的信息很少。14例狼疮性肾炎（LN）患者、13例IgA肾病（IgAN）患者和12名健康对照者参加了这项研究。采用实时定量聚合酶链反应检测尿沉积物中T-bet、gta -3、FOXP3和rig - 1 mRNA的表达。我们还研究了rig - 1在LN和IgAN患者肾组织标本中的表达。GATA-3、FOXP3和rig - 1的表达模式在三个研究组之间存在显著差异，T-bet的表达差异较小。LN患者的组织标本中观察到rig - 1的免疫荧光染色，而IgAN患者的组织标本中没有。尿沉渣中T-bet、GATA-3、FOXP3和rig - 1 mRNA的表达模式因诊断类型不同而不同。这些结果表明，测量这些靶基因的表达可能是一种有用的、无创的方法，用于临床监测和研究肾小球肾炎的发病机制。
Recent studies have suggested that gene expression studies using urinary sediment might be a non-invasive approach to assessing activity and pathogenesis in glomerulonephritis. However, little information is available regarding the mRNA expression patterns of functional molecules, such as T-bet, GATA-3, FOXP3, and retinoic acid-inducible gene-I (RIG-I), in urinary sediment, from patients with immunocomplex-mediated glomerulonephritis. Fourteen lupus nephritis (LN) patients, 13 IgA nephropathy (IgAN) patients, and 12 healthy controls were enrolled in the study. The mRNA expressions of T-bet, GATA-3, FOXP3 and RIG-I in urinary sediment were measured using real time quantitative polymerase chain reaction. We also studied the expression of RIG-I in kidney tissue specimens obtained from LN and IgAN patients. Significant differences in the expression patterns of GATA-3, FOXP3 and RIG-I, and marginal differences in T-bet expression, were observed between the three study groups. Immunofluorescent staining for RIG-I was observed in the tissue specimens from the LN patients, but not in those from the IgAN patients. The mRNA expression patterns of T-bet, GATA-3, FOXP3 and RIG-I in urinary sediment differ according to diagnostic category. These results suggest that the measurement of these target gene expressions might be a useful, non-invasive method for clinical monitoring and studying of pathogenesis in glomerulonephritis.