Nitrosamine-induced carcinogenesis. The alkylation of N-7 of guanine of nucleic acids of the rat by diethylnitrosamine, N-ethyl-N-nitrosourea and ethyl methanesulphonate.
Nitrosamine-induced carcinogenesis. The alkylation of N-7 of guanine of nucleic acids of the rat by diethylnitrosamine, N-ethyl-N-nitrosourea and ethyl methanesulphonate.
复制标题
亚硝胺诱发的致癌作用。
DOI：
10.1042/bj1250841
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发表时间：
1971
期刊：
影响因子：
--
通讯作者：
P. Magee
中科院分区：
文献类型：
--
作者：
P. F. Swann;P. Magee
1. 测定了二乙基亚硝胺、n -乙基-n -亚硝基脲和甲磺酸乙酯对体内大鼠组织核酸中鸟嘌呤N-7的甲基化程度。2. 所有化合物都产生了可测量的7-乙基鸟嘌呤。3. 单剂量的二乙基亚硝胺或n -乙基-n -亚硝基脲在大鼠体内产生肾脏肿瘤。三剂甲磺酸乙酯会产生肾脏肿瘤，但单剂则不会。4. 单剂量的二乙基亚硝胺在肾脏DNA中产生的鸟嘌呤N-7的甲基化量是n -乙基-n -亚硝基脲的两倍。两种化合物的单剂量均可诱发肾肿瘤，尽管其组织学类型不同。5. 单剂量甲基磺酸乙酯在肾脏DNA中产生的鸟嘌呤N-7的甲基化量是n -乙基-n -亚硝基脲的十倍，而不会产生肿瘤。6. 这些发现的相关性假设，细胞成分的烷基化是由亚硝基化合物诱导肿瘤的机制进行了讨论。
1. The extent of ethylation of N-7 of guanine in the nucleic acids of rat tissue in vivo by diethylnitrosamine, N-ethyl-N-nitrosourea and ethyl methanesulphonate was measured. 2. All compounds produced measurable amounts of 7-ethyl-guanine. 3. A single dose of diethylnitrosamine or N-ethyl-N-nitrosourea produced tumours of the kidney in the rat. Three doses of ethyl methanesulphonate produced kidney tumours, but a single dose did not. 4. A single dose of diethylnitrosamine produced twice as much ethylation of N-7 of guanine in DNA of kidney as did N-ethyl-N-nitrosourea. A single dose of both compounds induced kidney tumours, although of a different histological type. 5. A single dose of ethyl methanesulphonate produced ten times as much ethylation of N-7 of guanine in kidney DNA as did N-ethyl-N-nitrosourea without producing tumours. 6. The relevance of these findings to the hypothesis that alkylation of a cellular component is the mechanism of induction of tumours by nitroso compounds is discussed.