Dra/AfaE adhesin of uropathogenic Dr/Afa+ Escherichia coli mediates mortality in pregnant rats.
Dra/AfaE adhesin of uropathogenic Dr/Afa+ Escherichia coli mediates mortality in pregnant rats.
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尿路致病性 Dr/Afa 大肠杆菌的 Dra/AfaE 粘附素介导妊娠大鼠的死亡率。
DOI：
10.1128/iai.73.11.7597-7601.2005
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发表时间：
2005
影响因子：
3.1
通讯作者：
Nowicki,B
中科院分区：
文献类型：
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作者：
Wroblewska-Seniuk,K;Selvarangan,R;Hart,A;Pladzyk,R;Goluszko,P;Jafari,A;duMerle,L;Nowicki,S;Yallampalli,C;LeBouguénec,C;Nowicki,B
携带Dr/Afa家族粘附素的大肠杆菌经常导致人类妊娠期间的泌尿生殖道感染，并与妊娠大鼠的死亡率相关。粘附素的两种组分Dra/AfaE和Dra/AfaD被认为是毒力因子，负责细菌结合和内化。我们推测Dr/Afa+E. Dra/AfaE或Dra/AfaD这两种蛋白质之一介导的大肠杆菌。在本研究中，我们使用afaE和/或afaD突变体，研究了theafaE和afaD基因在宫内感染孕鼠死亡率中的作用。SD大鼠于妊娠第17天感染E. coli afaE+ afaD和afaE afaD+突变体。诊所E。大肠杆菌（afaE+afaD+）和茶黄素E afaD双突变株分别作为阳性和阴性对照。感染后24 h评价死亡率。在感染theafaE+afaD+菌株的组中观察到最高的孕产妇死亡率，其次是感染theafaE+ afaD菌株的组。死亡率呈剂量依赖性。TheafaE afaD双突变体即使在最高感染剂量下也不引起母体死亡。体内研究与侵袭测定相对应，其中茶afaE+菌株是最具侵袭性的（afaE+ afaD菌株>afaE+afaD+菌株），而茶afaE突变菌株（afaE afaD+和afaE afaD菌株）似乎是非侵袭性的。本研究首次表明，编码Dr/Afa粘附素AfaE亚基的theafaE基因参与了大鼠妊娠期感染的致死结果。与AfaE相关的这种致死作用与afaE +E的侵袭性相关。大肠杆菌体外培养。
Escherichia colibearing adhesins of the Dr/Afa family frequently causes urogenital infections during pregnancy in humans and has been associated with mortality in pregnant rats. Two components of the adhesin, Dra/AfaE and Dra/AfaD, considered virulence factors, are responsible for bacterial binding and internalization. We hypothesize that gestational mortality caused by Dr/Afa+E. coliis mediated by one of these two proteins, Dra/AfaE or Dra/AfaD. In this study, usingafaEand/orafaDmutants, we investigated the role of theafaEandafaDgenes in the mortality of pregnant rats from intrauterine infection. Sprague-Dawley rats, on the 17th day of pregnancy, were infected with theE. coli afaE+afaDandafaE afaD+mutants. The clinicalE. colistrain (afaE+afaD+) and theafaE afaDdouble mutant were used as positive and negative controls, respectively. The mortality rate was evaluated 24 h after infection. The highest maternal mortality was observed in the group infected with theafaE+afaD+strain, followed by the group infected with theafaE+afaDstrain. The mortality was dose dependent. TheafaE afaDdouble mutant did not cause maternal mortality, even with the highest infection dose. The in vivo studies corresponded with the invasion assay, where theafaE+strains were the most invasive (afaE+afaDstrain >afaE+afaD+strain), while theafaEmutant strains (afaE afaD+andafaE afaDstrains) seemed to be noninvasive. This study shows for the first time that theafaEgene coding for the AfaE subunit of Dr/Afa adhesin is involved in the lethal outcome of gestational infection in rats. This lethal effect associated with AfaE correlates with the invasiveness ofafaE+E. colistrains in vitro.