Tile-based self-assembly of a triple-helical polysaccharide into cell wall-like mesoporous nanocapsules.
Tile-based self-assembly of a triple-helical polysaccharide into cell wall-like mesoporous nanocapsules.
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DOI：
10.1039/c7nr02801f
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发表时间：
2017-07
期刊：
影响因子：
6.7
通讯作者：
Chaoxi Wu;Xiaoying Wang;Jianjing Wang;Zhen Zhang;Zhiping Wang;Yifei Wang;Shunqing Tang
中科院分区：
文献类型：
--
作者：
Chaoxi Wu;Xiaoying Wang;Jianjing Wang;Zhen Zhang;Zhiping Wang;Yifei Wang;Shunqing Tang
基于瓦片的自组装在构建三维DNA纳米结构中是一个强大的系统，但它很少被应用于其他螺旋生物聚合物。β-葡聚糖是一种具有免疫活性的天然聚合物，以三螺旋构象存在。在此，我们报道了β-葡聚糖，使用两种类型的短链酰基修饰后，可以自组装成瓷砖与两种溶剂的界面处的失活粘性末端。这些瓦片由横向排列的单层螺旋组成，当末端的一些酰基被去除时，粘性末端可以被激活;这些瓦片可以进一步包装成介孔纳米胶囊，其过程与粘性DNA瓦片包装成复杂的多面体纳米物体类似。发现这些纳米帽在RAW 264.7细胞模型中对抗原呈递细胞具有靶向作用。我们的研究表明，基于瓦片的自组装可以是螺旋生物聚合物的一般策略，充分利用这种策略，各种新的功能纳米结构将在未来变得容易。
Tile-based self-assembly is a robust system in the construction of three-dimensional DNA nanostructures but it has been rarely applied to other helical biopolymers. β-Glucan is an immunoactive natural polymer which exists in a triple helical conformation. Herein, we report that β-glucan, after modification using two types of short chain acyl groups, can self-assemble into tiles with inactivated sticky ends at the interface of two solvents. These tiles consist of a single layer of helices laterally aligned, and the sticky ends can be activated when a few acyl groups at the ends are removed; these tiles can further pack into mesoporous nanocapsules, in a similar process as the sticky DNA tiles pack into complex polyhedral nano-objects. These nanocapsules were found to have targeted effects to antigen presenting cells in a RAW264.7 cell model. Our study suggests that tile-based self-assembly can be a general strategy for helical biopolymers, and on fully exploiting this strategy, various new functional nanostructures will become accessible in the future.