Evidence of Differences between the Communities of Arbuscular Mycorrhizal Fungi Colonizing Galls and Roots of Prunus persica Infected by the Root-Knot Nematode Meloidogyne incognita
Evidence of Differences between the Communities of Arbuscular Mycorrhizal Fungi Colonizing Galls and Roots of Prunus persica Infected by the Root-Knot Nematode Meloidogyne incognita
复制标题
DOI：
10.1128/aem.05577-11
复制
发表时间：
2011-12-01
影响因子：
4.4
通讯作者：
Roldan, Antonio
中科院分区：
文献类型：
--
作者：
del Mar Alguacil, Maria;Torrecillas, Emma;Roldan, Antonio
丛枝菌根真菌（AMF）作为植物保护剂，具有减少或抑制线虫侵染的作用。然而，它从来没有被调查是否虫瘿产生的根线虫感染AMF定殖。本研究探讨了桃根内南方根结线虫侵染产生的虫瘿是否被AMF定殖。我们还确定了AMF的组成和生物多样性的变化介导的感染这种根结线虫。从感染M.用AMF特异性引物提取、扩增、克隆和测序了来自未感染植物根和来自未感染植物根的AMF特异性引物。利用小亚基（SSU）核糖体DNA（rDNA）数据集的系统发育分析揭示了22种不同的AMF序列类型（17种球囊霉属序列类型、3种副球囊霉属序列类型、1种盾孢霉属序列类型和1种无梗孢霉属序列类型）。AMF多样性最高的未感染的根，其次是感染的根和虫瘿。本研究表明，桃根中产生的虫瘿是由于感染M.结果表明，在南方根结线虫的根中，存在着与根中不同的丛枝菌根真菌群落，该群落隶属于拟丛枝菌根科和球囊霉科。虽然AMF在虫瘿中的功能仍然是未知的，我们假设它们作为保护剂对抗机会致病菌。
Arbuscular mycorrhizal fungi (AMF) play important roles as plant protection agents, reducing or suppressing nematode colonization. However, it has never been investigated whether the galls produced in roots by nematode infection are colonized by AMF. This study tested whether galls produced by Meloidogyne incognita infection in Prunus persica roots are colonized by AMF. We also determined the changes in AMF composition and biodiversity mediated by infection with this root-knot nematode. DNA from galls and roots of plants infected by M. incognita and from roots of noninfected plants was extracted, amplified, cloned, and sequenced using AMF-specific primers. Phylogenetic analysis using the small-subunit (SSU) ribosomal DNA (rDNA) data set revealed 22 different AMF sequence types (17 Glomus sequence types, 3 Paraglomus sequence types, 1 Scutellospora sequence type, and 1 Acaulospora sequence type). The highest AMF diversity was found in uninfected roots, followed by infected roots and galls. This study indicates that the galls produced in P. persica roots due to infection with M. incognita were colonized extensively by a community of AMF, belonging to the families Paraglomeraceae and Glomeraceae, that was different from the community detected in roots. Although the function of the AMF in the galls is still unknown, we hypothesize that they act as protection agents against opportunistic pathogens.