GERM-LINE TRANSFORMATION INVOLVING DNA FROM THE PERIOD LOCUS IN DROSOPHILA-MELANOGASTER - OVERLAPPING GENOMIC FRAGMENTS THAT RESTORE CIRCADIAN AND ULTRADIAN RHYTHMICITY TO PER0 AND PER- MUTANTS
GERM-LINE TRANSFORMATION INVOLVING DNA FROM THE PERIOD LOCUS IN DROSOPHILA-MELANOGASTER - OVERLAPPING GENOMIC FRAGMENTS THAT RESTORE CIRCADIAN AND ULTRADIAN RHYTHMICITY TO PER0 AND PER- MUTANTS
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DOI：
10.3109/01677068609106855
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发表时间：
1986-09-01
影响因子：
1.9
通讯作者：
HALL, JC
中科院分区：
文献类型：
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作者：
HAMBLEN, M;ZEHRING, WA;HALL, JC
涉及黑腹果蝇周期（per）时钟基因DNA片段的P元件介导的转化已表明该基因座的几个亚片段恢复了佩罗或per突变体的节律性。这些片段在与一个转录本互补的基因组区域中重叠，该转录本是一个4.5 kb的RNA，可能是每一个信息，因为它是必要的，并且（就从这个X染色体位点的表达而言）足以满足果蝇的昼夜节律。这至少也是求爱的雄性振动翅膀时产生的高频振荡所必需的。整个4.5 kb的转录是不必要的，而强大的节奏性，也似乎是不够的，在转化体中，野生型的行为表型。与紧邻4.5 kb物种来源的基因组区域同源的0.9 kb RNA在一天中的丰度振荡;但是在携带消除0.9 kb RNA振荡的佩罗突变的几个转化体中覆盖该转录物来源并不能恢复节律性。所有独立分离的突变都被相同的重叠的pR+衍生的DNA片段阵列覆盖，这意味着突变为突变的基因座的唯一部分与4.5-kb转录物互补。
P-element-mediated transformations involving DNA fragments from the period (per) clock gene of Drosophila melanogaster have shown that several subsegments of the locus restore rhythmicity to pero or per- mutants. Such fragments overlap in a genomic region complementary to one transcript, a 4.5-kb RNA which is probably the per message, in that it is necessary and (in terms of expression from this X-chromosomal locus) sufficient for the fly''s circadian rhythms. It is also at least necessary for the high-frequency oscillations normally produced by courting males as they vibrate their wings. The entirety of the 4.5-kb transcript is not necessary for rather strong rhythmicity; nor does it seem to be sufficient, in transformants, for wild-type behavioral phenotypes. A 0.9-kb RNA, homologous to a genomic region immediately adjacent to the source of the 4.5-kb species, oscillates in its abundance over the course of a day; but coverage of this transcript source in several transformants carrying a pero mutation.sbd.which eliminates the 0.9-kb RNA''s oscillation.sbd.does not restore rhythmicity. All of the independenlty isolated arrhythmic mutations tested were covered by the same array of overlapping per+-derived DNA fragments, implying that the only portion of the locus which has mutated to arrhythmicity is complementary to the 4.5-kb transcript.