Cholesterol Protein Interaction: Methods and Cholesterol Reporter Molecules
Cholesterol Protein Interaction: Methods and Cholesterol Reporter Molecules
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DOI：
10.1007/978-90-481-8622-8_1
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发表时间：
2010-01-01
期刊：
影响因子：
--
通讯作者：
Gimpl, Gerald
中科院分区：
文献类型：
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作者：
Gimpl, Gerald
胆固醇是真核细胞质膜的主要成分。它调节磷脂双分子层的物理状态，并在膜微结构域的形成中起关键作用。胆固醇也影响几种膜蛋白的活性，是类固醇激素和胆汁酸的前体。在这里，描述了用于探索蛋白质与胆固醇的结合和/或相互作用的方法。为此目的，各种胆固醇探针承载无线电，自旋，光亲和-或荧光标记目前是可用的。已证实的胆固醇结合分子有多烯化合物、胆固醇依赖的细胞溶解素、接受胆固醇作为底物的酶和具有胆固醇结合基序的蛋白质。本报告的主要主题是富胆固醇微域候选膜蛋白的定位，胆固醇蛋白相互作用的特异性问题，以及各种胆固醇探针在这些研究中的应用。
Cholesterol is a major constituent of the plasma membrane in eukaryotic cells. It regulates the physical state of the phospholipid bilayer and is crucially involved in the formation of membrane microdomains. Cholesterol also affects the activity of several membrane proteins, and is the precursor for steroid hormones and bile acids. Here, methods are described that are used to explore the binding and/or interaction of proteins to cholesterol. For this purpose, a variety of cholesterol probes bearing radio-, spin-, photoaffinity- or fluorescent labels are currently available. Examples of proven cholesterol binding molecules are polyene compounds, cholesterol-dependent cytolysins, enzymes accepting cholesterol as substrate, and proteins with cholesterol binding motifs. Main topics of this report are the localization of candidate membrane proteins in cholesterol-rich microdomains, the issue of specificity of cholesterol protein interactions, and applications of the various cholesterol probes for these studies.