Parvalbumin is expressed in glutamatergic and GABAergic corticostriatal pathway in mice
Parvalbumin is expressed in glutamatergic and GABAergic corticostriatal pathway in mice
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DOI：
10.1002/cne.20246
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发表时间：
2004-09-13
影响因子：
2.5
通讯作者：
Kosaka, T
中科院分区：
文献类型：
--
作者：
Jinno, S;Kosaka, T
皮质和纹状体之间的联系在控制和执行随意运动中起着至关重要的作用。在这里，我们专注于表达的钙结合蛋白小清蛋白（PV）在皮质纹状体通路。将荧光金注入纹状体引起压后皮质和体感皮质PV阳性神经元的逆行标记。PV阳性皮质纹状体投射神经元主要分布在第V层，但偶见于第II、III和VI层。逆标细胞的PV免疫反应性弱于非逆标细胞。虽然这是相当困难的，以分析形态的荧光金标记的神经元表现出PV免疫反应，其中一些显示出独特的顶端树突，被认为是锥体细胞。PV阳性皮质传入的主要靶点是同侧纹状体尾侧。接下来，我们测试了PV阳性的皮质纹状体投射神经元是否是GABA能的，因为以前的研究强调PV是皮质GABA能神经元的重要标志物。出乎意料的是，我们发现，大多数PV阳性皮质纹状体投射神经元谷氨酸脱羧酶（GAD）阴性，而其中一些是GAD阳性。最后，顺行示踪菜豆白细胞凝集素（PHA-L）注射到体感皮层导致许多PV阳性皮质纹状体终端囊泡谷氨酸转运蛋白1阳性，而一些PV阳性PHA-L标记的终端是GAD阳性。我们的研究结果提供了解剖学证据表明，在小鼠的多巴胺能和GABA能皮质纹状体通路的PV的表达，并建议，一个子集的皮质传入可能会产生一些抑制纹状体活动的影响。(C)2004 Wiley-Liss，Inc.
The connections between the cortex and the striatum are critically involved in control and execution of voluntary movements. Here we focused on the expression of calcium binding protein parvalbumin (PV) in the corticostriatal pathway. Injections of Fluorogold into the striatum gave rise to retrograde labeling of PV-positive neurons in the retrosplenial cortex and somatosensory cortex. The PV-positive corticostriatal projection neurons were mainly found in layer V, but occasionally seen in layers II, III, and VI. The PV immunoreactivity of retrogradely labeled cells was weaker than that of nonlabeled cells. Although it was rather difficult to analyze the morphology of Fluorogold-labeled neurons that exhibited PV immunoreactivity, some of them showed distinct apical dendrites and were considered pyramidal cells. The main target of PV-positive cortical afferents was the caudal striatum on the ipsilateral side. Next, we tested whether PV-positive corticostriatal projection neurons were GABAergic or not, because previous studies emphasized that PV was an important marker for cortical GABAergic neurons. Unexpectedly, we found that the majority of PV-positive corticostriatal projection neurons were glutamic acid decarboxylase (GAD)-negative, while some of them were GAD-positive. Finally, an anterograde tracer Phaseolus vulgaris leucoagglutinin (PHA-L) injection into the somatosensory cortex resulted in many PV-positive corticostriatal terminals that were vesicular glutamate transporter 1-positive, whereas some of the PV-positive PHA-L-labeled terminals were GAD-positive. Our results provide anatomical evidence for expression of PV in glutamatergic and GABAergic corticostriatal pathway in mice and suggest that a subset of cortical afferents may exert some inhibitory influence on striatal activity. (C) 2004 Wiley-Liss, Inc.