Influence of Oxidative Stress on Biocontrol Activity of Cryptococcus laurentii against Blue Mold on Peach Fruit
Influence of Oxidative Stress on Biocontrol Activity of Cryptococcus laurentii against Blue Mold on Peach Fruit
复制标题
氧化应激对劳伦隐球菌对桃青霉病生防活性的影响
DOI：
10.3389/fmicb.2017.00151
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发表时间：
2017-02-02
影响因子：
5.2
通讯作者：
Tian, Shiping
中科院分区：
文献类型：
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作者：
Zhang, Zhanquan;Chen, Jian;Tian, Shiping
化学杀菌剂在控制采后病害方面的局限性最近变得更加明显。利用拮抗微生物控制采后腐烂是一种很有前途的替代杀菌剂的方法。在以往的研究中，拮抗酵母菌Laurentii表现出了良好的生防效果和巨大的实际应用潜力。不利的条件，如氧化胁迫，限制了拮抗酵母的实际应用。在本研究中，我们研究了Laurentii对氧化应激的耐受性及其相关机制。结果表明，外源氧化胁迫对Laurentii幼虫的生存能力和生防效果有显著影响。H_2O_2诱导的氧化应激导致活性氧物种的积累。流式细胞仪分析的结果表明，细胞凋亡是氧化应激下劳伦蒂梭子蟹存活率下降的原因。通过抗氧化活性的测试，我们发现罗伦提可以利用酶系统来抵抗外源氧化应激。外源谷胱甘肽是一种非酶类抗氧化剂，外源谷胱甘肽的加入可显著提高劳伦蒂弧菌的氧化耐受性和生防效率。
The limitations of chemical fungicides for the control of postharvest diseases have recently become more apparent. The utilization of antagonistic microorganisms is a promising alternative to that of fungicides to control postharvest decay. In previous studies, the antagonistic yeast Cryptococcus laurentii has shown excellent effects of biocontrol and great potential for practical application. Adverse conditions, such as oxidative stress, limit the practical application of antagonistic yeast. In this study, we investigated the oxidative stress tolerance of C. laurentii and the associated mechanisms. The results indicated that exogenous oxidative stress has a significant effect on the viability and biocontrol efficiency of C. laurentii. H2O2-induced oxidative stress led to the accumulation of reactive oxygen species. The results of flow cytometric analysis suggested that apoptosis is responsible for the reduced survival rate of C. laurentii under oxidative stress. Using tests of antioxidant activity, we found that C. laurentii could employ enzymatic systems to resist exogenous oxidative stress. The addition of exogenous glutathione, a non-enzymatic antioxidant, to the media can significantly enhance oxidative tolerance and biocontrol efficiency of C. laurentii.