Role of Gap Junctional Intercellular Communication in Radiation-Induced Bystander Effects in Human Fibroblasts
Role of Gap Junctional Intercellular Communication in Radiation-Induced Bystander Effects in Human Fibroblasts
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DOI：
10.1667/rr3044
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发表时间：
2003-09
期刊：
影响因子：
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通讯作者：
C. Shao;Y. Furusawa;M. Aoki;K. Ando
中科院分区：
文献类型：
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作者：
C. Shao;Y. Furusawa;M. Aoki;K. Ando
摘要Shao，C.，Furusawa，Y.，青木，M.和Ando，K.缝隙连接细胞间通讯在人成纤维细胞辐射诱导的旁观者效应中的作用。Radiat. Res. 160，318-323（2003）。研究了细胞间隙连接通讯（GJIC）在碳离子束照射人成纤维细胞旁观者反应中的作用。结果发现，辐射剂量越低，每次穿越核的辐射诱发微核产量越高，表明存在旁观者效应。当用8-Br-cAMP处理细胞增强GJIC时，这种低剂量敏感性增加，但用DMSO（一种有效的活性氧（ROS）清除剂）处理细胞时，这种低剂量敏感性部分降低。此外，当细胞用100 µM的Lincidine（一种GJIC抑制剂）处理时，未观察到低剂量敏感性。DMSO可提高照射细胞的存活率，但cAMP和Lincoln对照射细胞的存活率无明显影响。另一方面，G1期阻滞在照射细胞中被检测到，并且它被cAMP增强。相比之下，这种逮捕减少或几乎消除了DMSO或linodine，分别，即使当细胞受到如此高的剂量照射，每个细胞平均收到五个核穿越。因此，旁观者反应发生后，低剂量和相对高剂量照射。我们的研究结果表明，GJIC和ROS都参与了辐射诱导的旁观者效应，但缝隙连接通道可能通过调节辐射诱导的信号因子的释放发挥重要作用。
Abstract Shao, C., Furusawa, Y., Aoki, M. and Ando, K. Role of Gap Junctional Intercellular Communication in Radiation-Induced Bystander Effects in Human Fibroblasts. Radiat. Res. 160, 318–323 (2003). Involvement of gap junctional intercellular communication (GJIC) in bystander responses of confluent human fibroblasts irradiated with a carbon-ion beam was investigated. It was found that the lower the radiation dose, the higher the yield of radiation-induced micronuclei per nuclear traversal, suggesting the existence of bystander effects. This low-dose sensitivity was increased when GJIC was enhanced by treating cells with 8-Br-cAMP, but it was partly reduced by treating cells with DMSO, an effective scavenger of reactive oxygen species (ROS). Moreover, no low-dose sensitivity was observed when cells were treated with 100 µM lindane, an inhibitor of GJIC. The survival of irradiated cells was increased by DMSO but was not influenced significantly by cAMP or lindane. On the other hand, G1-phase arrest was detected in the irradiated cells, and it was enhanced by cAMP. In contrast, this arrest was reduced or almost eliminated by DMSO or lindane, respectively, even when cells were irradiated with such a high dose that each cell received five nuclear traversals on average. Thus the bystander responses occurred after both low-dose and relatively high-dose irradiation. Our results indicated that both GJIC and ROS contributed to the radiation-induced bystander effect, but gap junctional channels might play an essential role by modulating the release of radiation-induced signaling factors.