Human milk contains elements that block binding of noroviruses to human histo-blood group antigens in saliva
Human milk contains elements that block binding of noroviruses to human histo-blood group antigens in saliva
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DOI：
10.1086/425159
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发表时间：
2004-11-15
影响因子：
6.4
通讯作者：
Pickering, LK
中科院分区：
文献类型：
--
作者：
Jiang, X;Huang, PW;Pickering, LK
诺如病毒（NV）识别人类组织血型抗原（HBGAs）作为受体。我们表征了人乳样品与代表VA 387、Norwalk、VA 207和MOH的重组病毒样颗粒的相互作用。对来自60名健康女性的乳汁样本进行了人类HBGAs及其阻断NVs结合的能力的测试。54例为分泌型（Se+），6例为非分泌型（Se-）。没有妇女在她们的乳汁样本中检测到A或B抗原。所有54个Se+牛奶样品，但6个Se-牛奶样品中没有阻断VA 387和诺瓦克病毒（Se+结合剂）与唾液样品的结合。所有6个路易斯阳性Se-乳样品阻断与VA 207的结合，并且Se+乳样品表现出可变的阻断活性。没有乳汁样品阻断MOH与A和B抗原的结合。分泌子和刘易斯抗原（而非A或B抗原）存在于人乳中，并负责阻断NV与受体结合，因此可能是保护母乳喂养的婴儿免受NV感染的诱饵受体。
Noroviruses (NVs) recognize human histo-blood group antigens (HBGAs) as receptors. We characterized the interaction of human milk samples with recombinant virus-like particles representing VA387, Norwalk, VA207, and MOH. Milk samples from 60 healthy women were tested for human HBGAs and for their ability to block the binding of NVs. Fifty-four women were secretors (Se+), and 6 were nonsecretors (Se-). No women had detectable A or B antigens in their milk samples. All 54 Se+ milk samples, but 0 of 6 Se- milk samples, blocked VA387 and Norwalk virus (Se+ binders) from binding to saliva samples. All 6 Lewis-positive Se- milk samples blocked binding to VA207, and variable blocking activities were exhibited by the Se+ milk samples. No milk samples blocked the binding of MOH to A and B antigens. Secretor and Lewis, but not A or B antigens, were present in human milk and were responsible for blocking NV binding to receptors and therefore are likely to be decoy receptors that protect breast-fed infants from NV infection.