Peri- and Postnatal Development of Cerebellar Compartments in the Mouse
Peri- and Postnatal Development of Cerebellar Compartments in the Mouse
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小鼠小脑室的围产期和产后发育
DOI：
10.1007/s12311-013-0450-6
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Hirofumi Fujita
中科院分区：
文献类型：
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作者：
I. Sugihara;Hirofumi Fujita
成年人小脑皮质分为纵向条纹，其中浦肯野细胞（PC）具有区室特异性分子表达谱。由于条纹隔室具有特定的传入和传出投射模式，它们是小脑功能定位的基础。这些隔间在发育过程中是如何形成的，通常还不清楚。本实验室最近的研究集中在小鼠小脑从胚胎17.5天（E17.5）开始的发育过程中，胚胎期的小脑区室化明显，到出生后6天（P6），成年型的小脑区室化开始建立。FoxP2是未成熟PC的标记分子之一，已被用于鉴定E17.5 PC。通过使用几种标记分子（PLCβ4、EphA4、Pcdh10和1NM 13转基因小鼠品系的报告分子）的不同表达谱，已经将PC亚群或簇彼此区分开。通过多重染色连续切片的三维重建对PC簇的空间组织进行分析，表明E17.5小脑中有54个PC簇。在E17.5到P6期间，各个簇在空间上重新排列成条带。综上所述，E17.5小脑中的聚集区室基本上是小脑皮质中成人型条纹区室的直接起源。
The adult cerebellar cortex is compartmentalized into longitudinal stripes, in which Purkinje cells (PCs) have compartment-specific molecular expression profiles. Since the striped compartments have specific afferent and efferent projection patterns, they underlie the functional localization of the cerebellum. How these compartments form during development is generally not understood. Our recent study focuses on development of the cerebellar compartmentalization from embryonic day 17.5 (E17.5), when embryonic clustered compartmentalization is evidently observed, to postnatal day 6 (P6), when adult-type striped compartmentalization begins to be established, in mouse. FoxP2, one of the marker molecules for immature PCs, has been used to identify E17.5 PCs. PC subsets or clusters have been distinguished from each other by using different expression profiles of several marker molecules (PLCβ4, EphA4, Pcdh10, and a reporter molecule of the 1NM13 transgenic mouse strain). Analysis of spatial organization of PC clusters by three-dimensional reconstruction from multiple-stained serial sections has indicated 54 PC clusters in the E17.5 cerebellum. Individual clusters are spatially rearranged into stripes in the period from E17.5 to P6. In summary, the clustered compartments in the E17.5 cerebellum are basically direct origin of the adult-type striped compartments in the cerebellar cortex.
zebrin II 的克隆揭示了其与醛缩酶 C 的同一性。
DOI：
10.1242/dev.120.8.2081
发表时间：
1994
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Ahn,AH;Dziennis,S;Hawkes,R;Herrup,K
通讯作者：
Herrup,K