Proteome signatures of inflammatory activated primary human peripheral blood mononuclear cells.
Proteome signatures of inflammatory activated primary human peripheral blood mononuclear cells.
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DOI：
10.1016/j.jprot.2012.07.012
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发表时间：
2012-12-05
影响因子：
3.3
通讯作者：
Gerner C
中科院分区：
文献类型：
--
作者：
Haudek-Prinz VJ;Klepeisz P;Slany A;Griss J;Meshcheryakova A;Paulitschke V;Mitulovic G;Stöckl J;Gerner C
蛋白质组分析是一种选择的方法，以确定标记蛋白的表达可能是某些疾病的特征。这些标记蛋白的形成源于疾病相关的病理生理过程。在健康个体中，外周血单个核细胞（PBMCs）以静止细胞状态循环，监测潜在的免疫相关事件，但具有以炎症方式快速有效地响应任何潜在病原体入侵的能力。这些细胞的活化很可能伴随着特征性的蛋白质组改变。因此，我们使用“自上而下”的2D-PAGE方法和“自下而上”的LC-MS /MS-based shotgun方法，通过蛋白质组分析研究了未经治疗和炎症激活的原代人pbmc。此外，我们纯化了原代人t细胞和单核细胞，并分别激活它们。比较分析使我们能够表征一个强大的蛋白质组特征，包括NAMPT和PAI2，这表明pbmc的激活。t细胞特异性炎症特征包括IRF-4、gbp1和以前未被表征的GBP5的翻译产物；相应的单核细胞特征包括PDCD5、IL1RN和IL1B。炎症激活的pbmc在某些疾病中的参与以及这些细胞对抗炎药物的反应可以通过这些标记蛋白的量化来评估。本文是特刊《集成组学》的一部分。►亚细胞分离技术的应用，包括细胞质、细胞核和分泌蛋白►代谢性标记的应用►不同细胞类型蛋白质组谱的比较►支持临床蛋白质组学研究的功能数据解释
Proteome profiling is the method of choice to identify marker proteins whose expression may be characteristic for certain diseases. The formation of such marker proteins results from disease-related pathophysiologic processes. In healthy individuals, peripheral blood mononuclear cells (PBMCs) circulate in a quiescent cell state monitoring potential immune-relevant events, but have the competence to respond quickly and efficiently in an inflammatory manner to any invasion of potential pathogens. Activation of these cells is most plausibly accompanied by characteristic proteome alterations. Therefore we investigated untreated and inflammatory activated primary human PBMCs by proteome profiling using a ‘top down’ 2D-PAGE approach in addition to a ‘bottom up’ LC–MS/MS-based shotgun approach. Furthermore, we purified primary human T-cells and monocytes and activated them separately. Comparative analysis allowed us to characterize a robust proteome signature including NAMPT and PAI2 which indicates the activation of PBMCs. The T-cell specific inflammation signature included IRF-4, GBP1and the previously uncharacterized translation product of GBP5; the corresponding monocyte signature included PDCD5, IL1RN and IL1B. The involvement of inflammatory activated PBMCs in certain diseases as well as the responsiveness of these cells to anti-inflammatory drugs may be evaluated by quantification of these marker proteins. This article is part of a Special Issue entitled: Integrated omics. ► Reproducible identification of marker proteins using 2D-gels and shotgun proteomics ► Application of sub-cellular fractionation including cytoplasmic, nuclear and secreted proteins ► Application of metabolic labeling of primary human cells ► Comparison of proteome profiles across different cell types ► Support of functional data interpretation of clinical proteomics studies
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DOI：
10.1016/j.bbapap.2003.09.015
发表时间：
2004-01-14
影响因子：
3.2
作者：
Dotzlaw, H;Schulz, M;Neeck, G
通讯作者：
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影响因子：
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影响因子：
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