Habitual physical activity is associated with the maintenance of neutrophil migratory dynamics in healthy older adults.
Habitual physical activity is associated with the maintenance of neutrophil migratory dynamics in healthy older adults.
复制标题
DOI：
10.1016/j.bbi.2016.02.024
复制
发表时间：
2016-08
期刊：
影响因子：
--
通讯作者：
Lord JM
中科院分区：
文献类型：
--
作者：
Bartlett DB;Fox O;McNulty CL;Greenwood HL;Murphy L;Sapey E;Goodman M;Crabtree N;Thøgersen-Ntoumani C;Fisher JP;Wagenmakers AJ;Lord JM
体力活动与老年人中性粒细胞迁移有关。作用的发生与炎症或表面受体表达无关。脂联素水平可能积极影响中性粒细胞迁移。高龄时中性粒细胞功能障碍是免疫衰老的标志。减少的迁移和杀菌活性增加了感染的风险。目前尚不清楚为什么中性粒细胞功能障碍会随着年龄的增长而发生。身体活动和有组织的锻炼被认为可以提高老年人的免疫功能。本研究的目的是全面评估中性粒细胞功能，并确定其与习惯性体力活动的关系。通过7天的加速度计佩戴，对211名老年人（67 ± 5岁）的体力活动水平进行了测定。20名最活跃的男性和女性在年龄和性别上与20名最不活跃的人相匹配。比较各组的中性粒细胞迁移、吞噬作用、氧化爆发、细胞表面受体表达、代谢健康参数和全身炎症。还将各组与10名年轻参与者（23 ± 4岁）进行了比较。最活跃的一组每天完成的步数是最不活跃的一组的两倍（p < 0.001），具有较低的BMI（p = 0.007）和体脂百分比（p = 0.029）。与最不活跃组相比，最活跃组中的中性粒细胞更好地向IL-8迁移（p < 0.05），并且与年轻组的中性粒细胞迁移相当（p > 0.05）。在调整BMI、体脂和血浆代谢标志物后，这些差异仍然存在，这些指标在组间存在差异。相关性分析显示，步数/天、高脂联素和低胰岛素与迁移能力呈正相关（p < 0.05）。趋化因子受体CXCR 1或CXCR 2的表达没有差异（两者p > 0.05）。最活跃组的CD 11b高于最不活跃组（p = 0.048）。在嗜中性粒细胞吞噬作用或对大肠杆菌的氧化爆发反应方面，活动组和年轻对照组之间没有观察到差异（p > 0.05）。年轻组具有较低浓度的IL-6、IL-8、MCP-1、CRP、IL-10和IL-13（全部p < 0.05），而两个老年组之间没有差异。这些数据表明，老年人中性粒细胞迁移功能受损，而不是杀菌功能受损，部分原因可能是体力活动减少。在健康老年人中，体力活动的2倍差异与中性粒细胞迁移动力学更好地保持相关。因此，增加习惯性体力活动可能有利于中性粒细胞介导的免疫。
Physical activity associates with neutrophil migration in the elderly. Effects occur independently of inflammation or surface receptor expression. Adiponectin levels may positively influence neutrophil migration. Dysfunctional neutrophils with advanced age are a hallmark of immunosenescence. Reduced migration and bactericidal activity increase the risk of infection. It remains unclear why neutrophil dysfunction occurs with age. Physical activity and structured exercise have been suggested to improve immune function in the elderly. The aim of this study was to assess a comprehensive range of neutrophil functions and determine their association with habitual physical activity. Physical activity levels were determined in 211 elderly (67 ± 5 years) individuals by 7-days of accelerometry wear. Twenty of the most physically active men and women were matched for age and gender to twenty of the least physically active individuals. Groups were compared for neutrophil migration, phagocytosis, oxidative burst, cell surface receptor expression, metabolic health parameters and systemic inflammation. Groups were also compared against ten young participants (23 ± 4 years). The most active group completed over twice as many steps/day as the least active group (p < 0.001), had lower BMI’s (p = 0.007) and body fat percentages (p = 0.029). Neutrophils migrated towards IL-8 better in the most active group compared to the least active (p < 0.05) and was comparable to that of the young (p > 0.05). These differences remained after adjusting for BMI, body fat and plasma metabolic markers which were different between groups. Correlations revealed that steps/day, higher adiponectin and lower insulin were positively associated with migratory ability (p < 0.05). There was no difference in expression of the chemokine receptors CXCR1 or CXCR2 (p > 0.05 for both). CD11b was higher in the most active group compared to the least active (p = 0.048). No differences between activity groups or young controls were observed for neutrophil phagocytosis or oxidative burst in response to Escherichia coli (p > 0.05). The young group had lower concentrations of IL-6, IL-8, MCP-1, CRP, IL-10 and IL-13 (p < 0.05 for all) with no differences between the two older groups. These data suggest that impaired neutrophil migration, but not bactericidal function, in older adults may be, in part, the result of reduced physical activity. A 2-fold difference in physical activity is associated with better preserved neutrophil migratory dynamics in healthy older people. As a consequence increasing habitual physical activity may be beneficial for neutrophil mediated immunity.