Circulation of a Novel Pattern of Infections by Enteric Adenovirus Serotype 41 among Children below 5 Years of Age in Kolkata, India
Circulation of a Novel Pattern of Infections by Enteric Adenovirus Serotype 41 among Children below 5 Years of Age in Kolkata, India
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DOI：
10.1128/jcm.01834-10
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发表时间：
2011-02-01
影响因子：
9.4
通讯作者：
Ramamurthy, Thandavarayan
中科院分区：
文献类型：
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作者：
Dey, Rakhi Sharma;Ghosh, Santanu;Ramamurthy, Thandavarayan
人腺病毒F亚属株（AdVF）是发展中国家急性胃肠炎病例中最常见的病毒株。亚属F由AdV血清型40（AdV-40）和AdV-41代表。大多数报告描述了AdV-41在急性胃肠炎病例中的优势。为了深入了解AdV-41菌株的流行病学和遗传变异，我们分析了1，053份腹泻儿童的粪便标本。ELISA和PCR检测肠道腺病毒40/41阳性分别为42例（4.0%）和56例（5.3%）。在1,305例无症状儿童中，ELISA和PCR分别检测到9例（0.7%）和22例（1.7%）肠道腺病毒40/40阳性。年龄分布显示，24个月以下儿童的发病率较高（90%）。AdV F感染在一年中以低频率观察，在2月和3月期间发生率增加。本研究中16个代表性AdV-41株六邻体基因的1至3个高变区（HVR）的序列分析证实了具有基因组类型簇1（GTC 1）/GTC 2的独特株系的循环。然而，这些菌株的纤维基因的序列分析证实了从轴区域的第15个重复基序的15个氨基酸缺失。两个GTC的存在反映了六邻体基因的HVR中氨基酸突变的积累。新的AdV-41株可能遵循与AdV-40相同的感染模式。有症状和无症状儿童的菌株之间的六邻体和纤维基因的序列没有显着的变化。我们的数据证实了在印度加尔各答5岁以下儿童中传播的AdV-41菌株具有新的模式。
Human adenovirus of strains subgenus F (AdV F) are the most common strains detected in acute gastroenteritis cases in developing countries. Subgenus F is represented by AdV serotype 40 (AdV-40) and AdV-41. Most of the reports have described the predominance of AdV-41 in acute gastroenteritis cases. To gain insight into the epidemiology and genetic variation of AdV-41 strains, we analyzed 1,053 stool specimens from children with diarrhea. Among them, 42 (4.0%) and 56 (5.3%) were positive for enteric adenovirus 40/41 by enzymelinked immunosorbent assay (ELISA) and PCR, respectively. For 1,305 asymptomatic children, 9 (0.7%) and 22 (1.7%) samples were positive for enteric adenovirus 40/40 by ELISA and PCR, respectively. The age distribution revealed a higher frequency (90%) in children < 24 months of age. AdV F infection was observed at a low frequency throughout the year, with an increased incidence occurring during February and March. Sequence analysis of one to three hypervariable regions (HVRs) of the hexon genes of 16 representative AdV-41 strains in this study confirmed circulation of a unique strain with genomic type cluster 1 (GTC1)/GTC2. However, sequence analysis of the fiber genes of these strains confirmed 15 amino acid deletions from the 15th repeat motif of the shaft region. The existence of two GTCs reflects the accumulation of amino acid mutations in the HVR of the hexon gene. The novel AdV-41 strain might follow the same infection pattern as AdV-40. There is no significant variation in the sequences of hexon and fiber genes among strains from symptomatic and asymptomatic children. Our data confirm the circulation of an AdV-41 strain with a novel pattern in Kolkata, India, among children below 5 years of age.