SPECTROPHOTOMETRIC METHOD FOR DETERMINING HYDROXYLAMINE REDUCTASE ACTIVITY IN HIGHER PLANTS
SPECTROPHOTOMETRIC METHOD FOR DETERMINING HYDROXYLAMINE REDUCTASE ACTIVITY IN HIGHER PLANTS
复制标题
DOI：
10.1021/ac60106a054
复制
发表时间：
1955-01-01
影响因子：
7.4
通讯作者：
BURRELL, RC
中科院分区：
文献类型：
--
作者：
FREAR, DS;BURRELL, RC
一种快速、简便的羟胺显色试验已被用于生物材料中微量羟胺的定量测定。羟胺与过量的8-羟基喹啉定量反应形成稳定的5，8-喹啉酮-5-（8-羟基-5-喹啉酰亚胺）。当在其吸收峰705 mg处进行分光光度测量时，该化合物在0 - 5 × 10 - 2毫摩尔羟胺/ml范围内遵守比尔定律。解决方案。该方法已成功地应用于大豆叶片中羟胺还原酶活性的测定。
A rapid, simple color test for hydroxylamine has been adapted for the quantitative determination of micromolar amounts of hydroxylamine in biological materials. The hydroxylamine reacts quantitatively with an excess of 8-quinolinol to form the stable 5, 8-quinolinequinone-5-(8-hydroxy-5-quinolylimide). When measured spec-trophotometrically at its absorption peak, 705 mg, this compound obeys Beer’s law over the range of 0 to 5 X 10" 2 millimole of hydroxylamine per ml. of solution. The procedure has been applied successfully for the determination of hydroxylamine reductase activity in soybean leaves.