Optimization of cell seeding on electrospun PCL-silk fibroin scaffolds
Optimization of cell seeding on electrospun PCL-silk fibroin scaffolds
复制标题
DOI：
10.1016/j.eurpolymj.2020.109838
复制
发表时间：
2020-07-05
影响因子：
6
通讯作者：
Cicha, Iwona
中科院分区：
文献类型：
--
作者：
Singh, Raminder;Eitler, David;Cicha, Iwona
用于再生医学的细胞生长支持材料的可用性有限。为了提高内皮细胞（HUVECs）和成纤维细胞在不同纤维直径的聚己内酯（PCL）静电纺毡上的附着和生长，以及在新型PCL/丝素蛋白（PCL/SF）共混纤维上的附着和生长，采用醋酸和甲酸的混合溶剂进行静电纺，制备了两种不同平均纤维直径的PCL毡，即微米和纳米级纤维。优化了静电纺丝方法制备PCL/SF共混纤维。比较了在不同纤维尺寸和组成的垫上二维培养的HUVEC和成纤维细胞的形态学（F-actin）、活力（钙黄绿素）和代谢活性（WST-8测定）。随后，使用3D径向磁性细胞接种制备圆柱形PCL/SF支架并用细胞定殖。与PCL微纤维相比，PCL和PCL/SF纳米纤维都为初始细胞粘附（第1天）提供了更好的支持。在第7天，在PCL/SF垫上观察到HUVEC和成纤维细胞的最高代谢活性。显微镜研究证实，在第7天，在PCL/SF上观察到对细胞生长的最佳支持。利用磁性纳米颗粒和三维径向磁性细胞接种成功地将PCL/SF管状支架定殖。总之，通过将SF与PCL共混获得的纤维联合收割机结合了PCL的机械优点和SF的改善的生物功能。静电纺丝PCL/SF纳米纤维代表了一种有前途的材料，可以产生具有增强细胞附着和组织再生潜力的平面和3D结构。
The availability of cell growth-supporting materials for regenerative medicine is limited. Here, we aimed to improve the attachment and growth of endothelial cells (HUVECs) and fibroblasts on electrospun poly(epsilon-caprolactone) (PCL) mats with different fiber diameters and on newly-established PCL and silk fibroin (PCL/SF) blended fibers obtained by using benign solvents (mixture of acetic acid and formic acid) for electrospinning.PCL mats were produced in two different average fiber diameters, namely micro and nano range. The electrospinning method was optimized to produce PCL/SF blended fibers. Morphology (F-actin), viability (calcein) and metabolic activity (WST-8 assay) of HUVECs and fibroblasts cultured in 2D on the mats of different fiber size and composition were compared. Subsequently, cylindrical PCL/SF scaffolds were produced and colonized with cells using 3D radial magnetic cell seeding.Both PCL and PCL/SF nanofibers provided better support for initial cell adhesion (day 1) compared with PCL microfibers. At day 7, the highest metabolic activity in HUVECs and fibroblasts was observed on PCL/SF mats. Microscopic studies confirmed that on day 7, the best support for cell growth was observed on PCL/SF. Tubular PCL/SF scaffolds were successfully colonized using magnetic nanoparticles and 3D radial magnetic cell seeding.Taken together, the fibers obtained by blending SF with PCL combine the mechanical benefits of PCL with improved biological functionality of SF. Electrospun PCL/SF nanofibers represent a promising material to produce both flat and 3D structures with potential for enhanced cell attachment and tissue regeneration.