Profiling of Phenols in Human Fecal Water after Raspberry Supplementation
Profiling of Phenols in Human Fecal Water after Raspberry Supplementation
复制标题
DOI：
10.1021/jf1017143
复制
发表时间：
2010-10-13
影响因子：
6.1
通讯作者：
Rowland, Ian R.
中科院分区：
文献类型：
--
作者：
Gill, Chris I. R.;McDougall, Gorpon J.;Rowland, Ian R.
粪便水样中的酚类成分，从10个自由生活的人类受试者没有明显的饮食限制进行了监测之前和之后的乌藨子泥摄入量（200克/天，4天），使用气相色谱-质谱法。摄入后，所有受试者中没有单一酚类成分增加，但大多数受试者的苯乙酸（7/10）、4-羟基苯乙酸（6/10）、3-羟基苯乙酸（5/10）、3-苯基丙酸和3-（4-羟基苯基）丙酸显著升高。8/10例受试者的3，4-二羟基苯甲酸水平升高，6例受试者显著升高（p < 0.05），另2例受试者未显著降低。此外，与大多数其他粪便代谢物不同，增加始终>2倍。这种代谢物可能是代表增加结肠剂量的花青素。个体之间的结肠微生物群差异很大，补充乌藨子不会对结肠细菌的分布产生任何统计学显著的改变，也没有发现一种常见的模式来解释粪便水酚类分布中观察到的个体间差异。
The phenolic compositions of fecal water samples from ten free-living human subjects without marked dietary restrictions were monitored before and after intake of raspberry puree (200 g/day, 4 days) using gas chromatography-mass spectrometry. No single phenolic component was increased in all subjects after intake, but a majority of subjects had significant elevations in phenylacetic acid (7/10), 4-hydroxyphenylacetic acid (6/10), 3-hydroxyphenylacetic acid (5/10), 3-phenylpropionic acid and 3-(4-hydroxyphenyl)propionic acid. The levels of 3,4-dihydroxbenzoic acid were elevated in 8/10 subjects, significantly for 6 subjects (p < 0.05), and not significantly reduced in the other 2 subjects. In addition, unlike most other fecal metabolites, the increase was always >2-fold. This metabolite may be representative of the increased colonic dose of cyanidin anthocyanins. The colonic microbiota varied greatly between individuals, and supplementation with raspberries did not produce any statistically significant alterations in the profile of colonic bacteria, nor was a common pattern revealed to account for the interindividual variations observed in the fecal water phenolic profiles.