Structure, functional properties and iron bioavailability of Pneumatophorus japonicus myoglobin and its glycosylation products
Structure, functional properties and iron bioavailability of Pneumatophorus japonicus myoglobin and its glycosylation products
复制标题
DOI：
10.1016/j.ijbiomac.2021.01.138
复制
发表时间：
2021-01-27
影响因子：
8.2
通讯作者：
Yang, Wenge
中科院分区：
文献类型：
--
作者：
Tan, Beibei;Sun, Bolun;Yang, Wenge
开发安全有效的铁补充剂对于减轻缺铁性贫血（IDA）非常重要。肌红蛋白（MB）是富含生物利用铁的血红素蛋白质。中国重要的经济鱼类肺炎肺炎（P. japonicus）在其加工过程中通常被丢弃的深色肉中的MB水平很高。本研究旨在确定从japonicus提取的MB的结构，物理化学特性和铁生物利用度。同时，评估了糖基化（通常应用化学修饰的蛋白质）对这些参数的作用。使用盒子设计，获得了MB-壳聚糖糖基化的最佳条件：45.07摄氏度，pH 6.10和MB/壳聚糖质量比为6.29。研究了糖基化MB（MB-GLY）的结构和功能特性。与原始MB相比，MB-GLY获得了更有序的二级结构。发现MB-GLY的表面疏水性与升高水溶性的观察结果一起降低。此外，糖基化增强了MB抗氧化能力，并提高了其在酶消化系统中的稳定性。关于铁生物利用度，MB铁的细胞摄取明显高于FESO4，并通过糖基化进一步升高。这些结果为开发基于MB的铁补充剂提供了基础，从而促进了鱼类加工行业废物的利用。 （c）2021 Elsevier B.V.保留所有权利。
Developing safe and efficient iron supplements is significant for the alleviation of iron-deficient anemia (IDA). Myoglobin (Mb) is a heme-protein rich in bioavailable iron. Pneumatophorus japonicus (P. japonicus), one important economic fish in China, contain a high Mb level in its dark meat normally discarded during processing. The present study aimed to determine the structure, physicochemical properties, and iron bioavailability of Mb extracted from P. japonicus. Meanwhile, the effects of glycosylation, a commonly applied chemical modification of proteins, on these parameters were evaluated. Using Box-Behnken design, the optimal conditions for Mb-chitosan glycosylation were obtained: 45.07 degrees C, pH 6.10 and Mb/chitosan mass ratio of 6.29. The structure and functional properties of the glycosylated Mb (Mb-gly) were investigated. Compared with the original Mb, Mb-gly obtained a more ordered secondary structure. The surface hydrophobicity of Mb-gly was found to be decreased together with the observations of elevated water solubility. Moreover, glycosylation enhanced the Mb antioxidant capacity, and improved its stability in enzymatic digestion system. Regarding to the iron bioavailability, the cellular uptake of Mb-iron was significantly higher than FeSO4, and further elevated by glycosylation. These results provided a basis for the development of Mb-based iron supplements, promoting the utilization of fish-processing industries wastes. (C) 2021 Elsevier B.V. All rights reserved.