Identification of first exfoliative toxin in Staphylococcus pseudintermedius
Identification of first exfoliative toxin in Staphylococcus pseudintermedius
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DOI：
10.1111/j.1574-6968.2009.01823.x
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发表时间：
2009-12-01
影响因子：
2.1
通讯作者：
Fukuyasu, Tsuguaki
中科院分区：
文献类型：
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作者：
Futagawa-Saito, Keiko;Makino, Shinichiroh;Fukuyasu, Tsuguaki
已知金黄色葡萄球菌、猪葡萄球菌和产色葡萄球菌通过产生剥脱性毒素（exfoliative toxins，简称exfoliative toxins）引起人或动物的皮肤感染。假中间葡萄球菌也可引起犬化脓性脓疡，但没有剥脱性毒素或类似毒素的报道。用靶向S. aureus和SHETB。hyicus的基因组测序和随后的染色体步移鉴定了一个新基因，命名为exi（exfoliative toxin of pseudintermedius）。伪中间体EXI与脱落毒素具有显著的同源性（43-68%同一性），特别是与ETB（67.1%）、ETD（67.9%）和SHETB（65.1%）。系统进化分析表明，EXI与ETB的亲缘关系较近，自展值为80%。用来自培养物上清液或重组EXI的粗蛋白质注射的新生小鼠表现出严重的水泡和/或特征性皮肤剥脱。斑点杂交法检测出的exi阳性率为23.3%（10/43）。从犬脓毒症中分离的假中间体。本文报道的EXI是第一个在S.伪中间体
Staphylococcus aureus, Staphylococcus hyicus, and Staphylococcus chromogenes are known to cause skin infections in human or animals by producing exfoliative toxins (ETs). Staphylococcus pseudintermedius can also cause canine pyoderma, but no exfoliative toxins or similar toxins have been reported. PCR with degenerate primers targeted to the conserved regions in ETA, ETB, and ETD from S. aureus and SHETB from S. hyicus, and subsequent chromosome walking identified a novel gene, designated as exi (exfoliative toxin of pseudintermedius) in S. pseudintermedius. EXI had significant homologies with the exfoliative toxins (43-68% identity), particularly with ETB (67.1%), ETD (67.9%), and SHETB (65.1%). Phylogenetic analysis showed close relation between EXI and ETB with a bootstrap value of 80%. Neonatal mice injected with the crude proteins from the culture supernatant or recombinant EXI showed gross blisters and/or characteristic skin exfoliation. The prevalence of exi assessed by dot-blot hybridization was 23.3% (10/43) in S. pseudintermedius isolates from canine pyoderma. The EXI reported herein is the first exfoliative toxin identified in S. pseudintermedius.