No evidence for an association between common nonsynonymous polymorphisms in Delta and bristle number variation in natural and laboratory populations of Drosophila melanogaster
No evidence for an association between common nonsynonymous polymorphisms in Delta and bristle number variation in natural and laboratory populations of Drosophila melanogaster
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DOI：
10.1534/genetics.166.1.291
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发表时间：
2004-01-01
期刊：
影响因子：
3.3
通讯作者：
Long, AD
中科院分区：
文献类型：
--
作者：
Genissel, A;Pastinen, T;Long, AD
我们测试了这样的假设：对于一大群野生捕获的果蝇和先前描述的实验室品系，Notch 信号通路 Delta 配体中自然发生的非同义变异导致果蝇胸胸膜和/或腹部刚毛数量的持续变化。我们对 16 个天然存在的染色体的 Delta 转录区域进行了测序，观察到 65 个 SNP，包括 7 个非同义 SNP (nsSNP)。使用基于 OLA 的方法对 2060 个野生捕获的果蝇进行了鉴定的 nsSNP 和 6 个其他常见 SNP（均位于外显子 6 和 3' UTR）中的基因分型，并通过 DNA 测序对另外 38 个天然染色体进行了基因分型。通过排列测试评估，基因分型的 nsSNP 均未与鬃毛数量的自然变异显着相关。大型自然队列中每个基因型 SNP 的加性遗传方差的 95% 上限为
We test the hypothesis that naturally occurring nonsynonymous variants in the Delta ligand of the Notch signaling pathway contribute to standing variation in sternopleural and/or abdominal bristle number in Drosophila melanogaster, for both a large cohort of wild-caught flies and previously described laboratory lines. We sequenced the transcribed region of Delta for 16 naturally occurring chromosomes and 65 SNPs, including 7 nonsynonymous SNPs (nsSNPs), were observed. Identified nsSNPs and 6 additional common SNPs, all located in exon 6 and the 3' UTR, were genotyped in 2060 wild-caught flies using an OLA-based methodology and genotyped in 38 additional natural chromosomes via DNA sequencing. None of the genotyped nsSNPs were significantly associated with natural variation in bristle number as assessed by a permutation test. A 95% upper bound on the additive genetic variance attributable to each genotyped SNP in the large natural cohort is