Effects of a small, volatile bacterial molecule on Pseudomonas aeruginosa bacteria using whole cell high-resolution magic angle spinning nuclear magnetic resonance spectroscopy and genomics.
Effects of a small, volatile bacterial molecule on Pseudomonas aeruginosa bacteria using whole cell high-resolution magic angle spinning nuclear magnetic resonance spectroscopy and genomics.
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DOI：
10.3892/ijmm.2018.3760
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发表时间：
2018-10
影响因子：
5.4
通讯作者：
Tzika AA
中科院分区：
文献类型：
--
作者：
Righi V;Constantinou C;Kesarwani M;Rahme LG;Tzika AA
在本研究中，高分辨率魔角旋转（HRMAS）核磁共振（NMR）光谱应用于活铜绿假单胞菌（PA）细菌细胞，以确定这种机会性革兰氏阴性人类病原体的代谢组，特别是它对挥发性芳香族低分子量信号分子2-氨基苯乙酮（2-AA）的反应。多维HRMAS NMR是一种很有前途的方法，可用于确定活的完整细菌细胞的体内代谢组; 2-AA由PA产生，并引发促进体外和体内（动物）模型中慢性感染表型的表型的出现。在本研究中，我们应用一维和二维质子（1H）HRMAS NMR PA细胞生长与或不与2-AA，以检查之间的关联的代谢产物和细胞过程中响应2-AA。我们还比较了有或没有2-AA生长的PA细胞的全基因组转录组谱，发现2-AA促进PA细胞的深刻代谢变化。通过比较全基因组转录组谱和代谢组学分析，我们证明了2-AA深刻地重新编程了细胞的基因表达和代谢谱。我们的体内1H HRMAS NMR光谱可能被证明是一个有用的工具，在基因功能的验证，致病机制的研究，微生物菌株的功能/临床组的分类和测试的抗菌剂。
In the present study, high-resolution magic-angle spinning (HRMAS) nuclear magnetic resonance (NMR) spectroscopy was applied to live Pseudomonas aeruginosa (PA) bacterial cells to determine the metabolome of this opportunistic Gram-negative human pathogen, and in particular, its response to the volatile aromatic low molecular weight signaling molecule, 2-aminoacetophenone (2-AA). Multi-dimensional HRMAS NMR is a promising method which may be used to determine the in vivo metabolome of live intact bacterial cells; 2-AA is produced by PA and triggers the emergence of phenotypes that promote chronic infection phenotypes in in vitro and in vivo (animal) models. In the present study, we applied one-dimensional and two-dimensional proton (1H) HRMAS NMR to PA cells which were grown with or without 2-AA in order to examine the associations between metabolites and cellular processes in response to 2-AA. We also compared whole-genome transcriptome profiles of PA cells grown with or without 2-AA and found that 2-AA promoted profound metabolic changes in the PA cells. By comparing the whole-genome transcriptome profiles and metabolomic analysis, we demonstrated that 2-AA profoundly reprogramed the gene expression and metabolic profiles of the cells. Our in vivo 1H HRMAS NMR spectroscopy may prove to be a helpful tool in the validation of gene functions, the study of pathogenic mechanisms, the classification of microbial strains into functional/clinical groups and the testing of anti-bacterial agents.
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DOI：
10.2174/1874285800903010128
发表时间：
2009-08-13
期刊：
The open microbiology journal
影响因子：
--
作者：
Bazire A;Diab F;Taupin L;Rodrigues S;Jebbar M;Dufour A
通讯作者：
Dufour A
影响因子：
4.4
作者：
DSOUZAAULT, MR;SMITH, LT;SMITH, GM
通讯作者：
SMITH, GM
影响因子：
6.7
作者：
Kesarwani, Meenu;Hazan, Ronen;Rahme, Laurence G.
通讯作者：
Rahme, Laurence G.
影响因子：
1.6
作者：
MEIBOOM, S;GILL, D
通讯作者：
GILL, D
影响因子：
168.9
作者：
KOCH, C;HOIBY, N
通讯作者：
HOIBY, N