Effect of lectins on migration of the corneal epithelium.
Effect of lectins on migration of the corneal epithelium.
复制标题
凝集素对角膜上皮迁移的影响。
DOI：
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发表时间：
1980
影响因子：
4.4
通讯作者：
R. Anderson
中科院分区：
文献类型：
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作者：
I. Gipson;R. Anderson
将中央角膜擦伤为 3 mm 的大鼠角膜在四种植物凝集素：刀豆球蛋白 A (ConA)、麦芽凝集素 (WGA)、莲花凝集素和大豆凝集素 (SBA) 的存在下进行器官培养，20 和 50 微克/ml 培养基中的 ConA 会减慢上皮迁移。 20 和 50 微克/毫升培养基的 WGA 完全抑制迁移。 20 微克和 50 微克浓度的刀豆蛋白 A 以及 20 微克浓度的 WGA 的作用都是可逆的。 Lotus 凝集素和 SBA 在浓度高达并包括 50 微克/ml 培养基时不会影响运动性。然而，SBA 结合的特定糖 N-乙酰半乳糖胺确实减缓了上皮迁移。这项研究表明，阻断细胞表面和/或基底膜上糖蛋白的葡萄糖、甘露糖和葡萄糖胺糖部分可抑制角膜擦伤愈合过程中的上皮细胞迁移。
Rat corneas with 3 mm central corneal abrasions were organ-cultured in the presence of four plant lectins, concanavalin A (ConA), wheat germ agglutinin (WGA), lotus lectin, and soybean agglutinin (SBA), ConA at 20 and 50 micrograms/ml of culture medium slowed epithelial migration. WGA at 20 and 50 micrograms/ml of medium completely inhibited migration. The effects of the ConA at both 20 and 50 micrograms concentrations and of WGA at 20 micrograms were reversible. Lotus lectin and SBA did not effect motility at concentrations up to and including 50 micrograms/ml of medium. The specific sugar to which SBA binds, N-acetyl-galactosamine, did however, slow epithelial migrations. This study demonstrates that blocking glucose, mannose, and glucosamine sugar moities of glycoproteins on the cell surface and/or basement membrane inhibits epithelial cell migration during healing of corneal abrasions.