B cells establish, but do not maintain, long-lived murine anti-peanut IgE(a).
B cells establish, but do not maintain, long-lived murine anti-peanut IgE(a).
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DOI：
10.1111/cea.12715
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发表时间：
2016-04
期刊：
影响因子：
--
通讯作者：
Dreskin SC
中科院分区：
文献类型：
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作者：
Moutsoglou DM;Dreskin SC
Peanut allergy has been reported to be transferred to tolerant recipients through organ and bone marrow transplantation. The roles T and B cells play in establishing, and the roles B cell subsets play in maintaining lifelong anti-peanut IgE levels are unknown. To determine the cellular requirements for the transfer of murine peanut allergy and to determine the role CD20+ cells play in maintaining long-lived anti-peanut IgE levels. We developed a novel adoptive transfer model to investigate the cellular requirements for transferring murine peanut allergy. We also treated peanut-allergic mice with anti-CD20 antibody and measured IgE levels throughout treatment. Purified B220+ cells from peanut-allergic splenocytes and purified CD4+ cells from naïve splenocytes are the minimal requirements for the adoptive transfer of peanut allergy. Prolonged treatment of allergic mice with anti-CD20 antibody results in significant depletion of B cell subsets but does not affect anti-peanut IgE levels, symptoms, or numbers of IgE antibody secreting cells in the bone marrow. Adoptive transfer of bone marrow and spleen cells from allergic donors treated with anti-CD20 antibody does not result in the transfer of peanut allergy in naïve recipients, demonstrating that anti-CD20 antibody treatment depletes B cells capable of differentiating into peanut-specific IgE antibody secreting cells. Peanut allergy can be established in a naïve hosts with B220+ cells from peanut-allergic donors and CD4+ cells from peanut-naïve donors. However, long-term depletion of B220+ cells with anti-CD20 antibody does not affect anti-peanut IgE levels. These results highlight a novel role for B cells in the development of peanut allergy and provide evidence that long-lived anti-peanut IgE levels may be maintained by long-lived antibody secreting cells.
