Alcohol use disorders affect antimicrobial proteins and anti-pneumococcal activity in epithelial lining fluid obtained via bronchoalveolar lavage.
Alcohol use disorders affect antimicrobial proteins and anti-pneumococcal activity in epithelial lining fluid obtained via bronchoalveolar lavage.
复制标题
酒精使用障碍会影响通过支气管肺泡灌洗获得的上皮衬液中的抗菌蛋白和抗肺炎球菌活性。
DOI：
10.1093/alcalc/agq045
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发表时间：
2010
期刊：
影响因子：
--
通讯作者：
Cooper,Jacinta
中科院分区：
文献类型：
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作者：
Burnham,EllenL;Gaydos,Jeanette;Hess,Edward;House,Robert;Cooper,Jacinta
目的：我们的总体目标是检查酒精使用障碍(AUDS)患者通过支气管肺泡灌洗(BAL)获得的上皮衬里液体(ELF)的特征是否与他们患肺炎球菌肺炎的易感性有关。我们试图从AUDS受试者和匹配的对照组中比较脱细胞人BAL的抗肺炎球菌活性。进一步检测两组BAL溶菌酶活性和乳铁蛋白浓度的差异，以确定AUDS对这些抗菌蛋白的影响。方法：对AUDS患者和匹配的对照组进行BAL。将不同浓度的无细胞BAL用于2型肺炎链球菌的体外杀灭试验，确定每毫升总BAL蛋白对微生物的杀灭率。测定受试者和对照组的BAL中溶菌酶活性和乳铁蛋白浓度。结果：AUD组(n=15)和对照组(n=0.10)的吸烟史基本一致。使用混合效应模型，在BAL蛋白浓度范围内，AUD受试者的BAL液对肺炎球菌的杀灭效果往往较差。此外，AUD组的溶菌酶活性和乳铁蛋白浓度显著低于AUD组。结论：AUDS患者肺炎球菌肺炎的易感性可能部分是通过酒精对ELF中包括抗菌蛋白的物质的影响而实现的。通过对人类BAL液的调查，澄清AUDS环境中ELF抗菌蛋白的组成和活性，有助于确定它们对这些人肺部感染的易感性的贡献。
Aims: Our overall objective was to examine whether characteristics of epithelial lining fluid (ELF) from subjects with alcohol use disorders (AUDs) obtained via bronchoalveolar lavage (BAL) contribute to their predisposition to pneumococcal pneumonia. We sought to compare the anti-pneumococcal activity of acellular human BAL from subjects with AUDs to matched controls. Further, differences in BAL lysozyme activity and lactoferrin concentrations between these two groups were examined to determine the effect of AUDs on these antimicrobial proteins.Methods:BAL was performed in subjects with AUDs and matched controls. Acellular BAL was used at varying concentrations in anin vitrokilling assay ofStreptococcus pneumoniae, type 2, and the percent kill of organisms per microgram per milliliter total BAL protein was ascertained. Lysozyme activity and lactoferrin concentrations were measured in BAL from subjects and controls at measured concentrations of BAL protein.Results:AUD subjects (n= 15) and controls (n= 10) were enrolled in these investigations who were balanced in terms of smoking history. Using a mixed effect model, across the range of BAL protein concentrations, killing of pneumococcus tended to be less potent with BAL fluid from AUD subjects. Additionally, lysozyme activity and lactoferrin concentrations were significantly lower in the AUD group.Conclusions:The predisposition for pneumococcal pneumonia among those with AUDs may be in part mediated through effects of alcohol on substances within ELF that include antimicrobial proteins. Clarifying the composition and activity of ELF antimicrobial proteins in the setting of AUDs via investigations with human BAL fluid can help establish their contribution to the susceptibility for pulmonary infections in these individuals.