Solution structure of the coxsackievirus and adenovirus receptor domain 1.
Solution structure of the coxsackievirus and adenovirus receptor domain 1.
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DOI：
10.1021/bi035490x
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发表时间：
2004-02
期刊：
影响因子：
2.9
通讯作者：
Shaokai Jiang;A. Jacobs;T. Laue;M. Caffrey
中科院分区：
文献类型：
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作者：
Shaokai Jiang;A. Jacobs;T. Laue;M. Caffrey
柯萨奇病毒和腺病毒受体（CAR）介导柯萨奇病毒B（CVB）和腺病毒（Ad）的进入。CAR的正常细胞功能在多种组织类型中表达，被认为涉及发育中大脑中的嗜同性细胞粘附。CAR的胞外结构域由两个免疫球蛋白（IG）结构域组成，称为CAR-D1和CAR-D2。通过沉降速度显示CAR-D1在pH 3.0时为单体。已通过NMR光谱法在pH 3.0下测定了单体CAR-D1的溶液结构和动态性质。根据单体结构讨论了CAR-D1单体-二聚体平衡的决定因素以及CVB和Ad在CAR上的结合位点。
The coxsackievirus and adenovirus receptor (CAR) mediates entry of coxsackievirus B (CVB) and adenovirus (Ad). The normal cellular function of CAR, which is expressed in a wide variety of tissue types, is thought to involve homophilic cell adhesion in the developing brain. The extracellular domain of CAR consists of two immunoglobulin (Ig) domains termed CAR-D1 and CAR-D2. CAR-D1 is shown by sedimentation velocity to be monomeric at pH 3.0. The solution structure and the dynamic properties of monomeric CAR-D1 have been determined by NMR spectroscopy at pH 3.0. The determinants of the CAR-D1 monomer-dimer equilibrium, as well as the binding site of CVB and Ad on CAR, are discussed in light of the monomer structure.