Multiepitope Templates Imprinted Particles for the Simultaneous Capture of Various Target Proteins
Multiepitope Templates Imprinted Particles for the Simultaneous Capture of Various Target Proteins
复制标题
用于同时捕获各种靶蛋白的多表位模板印迹颗粒
DOI：
10.1021/acs.analchem.6b01247
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发表时间：
2016
影响因子：
7.4
通讯作者：
Zhang Yukui
中科院分区：
文献类型：
--
作者：
Yang Kaiguang;Li Senwu;Liu Jianxi;Liu Lukuan;Zhang Lihua;Zhang Yukui
为实现对多种目标蛋白的同时捕获，采用相转化聚醚砜（PES）自组装技术制备了多表位模板印迹粒子。本文以人血浆中丰度最高的三种蛋白质血清白蛋白、免疫球蛋白G和转铁蛋白为靶蛋白，合成其N端肽段作为表位模板。在二甲基乙酰胺中对3个表位和PES进行预组装后，在水中通过自组装形成多表位模板印迹粒子，实现了对人血浆中3种目标蛋白的高选择性同时识别。结合动力学研究表明，该印迹体系对三种表位模板的吸附机理与单表位印迹聚合物相同。这些结果表明，我们提出的多表位模板印迹策略可能会打开一个新的时代，人工抗体，以实现识别多种目标的同时。
To achieve the simultaneous capture of various target proteins, the multiepitope templates imprinted particles were developed by phase inversion-based poly(ether sulfone) (PES) self-assembly. Herein, with the top three high-abundance proteins in the human plasma, serum albumin, immunoglobulin G, and transferrin, as the target proteins, their N-terminal peptides were synthesized as the epitope templates. After the preorganization of three epitopes and PES in dimethylacetamide, the multiepitope templates imprinted particles were formed in water through self-assembly, by which the simultaneous recognition of three target proteins in human plasma was achieved with high selectivity. Furthermore, the binding kinetics study proved that the adsorption mechanism in this imprinting system toward three epitope templates was the same as that on the single-epitope imprinting polymer. These results demonstrate that our proposed multiepitope templates imprinting strategy might open a new era of artificial antibodies to achieve the recognition of various targets simultaneously.