Phospholipase C is required for the control of stomatal aperture by ABA
Phospholipase C is required for the control of stomatal aperture by ABA
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DOI：
10.1046/j.1365-313x.2003.01698.x
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发表时间：
2003-04-01
期刊：
影响因子：
7.2
通讯作者：
Gray, JE
中科院分区：
文献类型：
--
作者：
Hunt, L;Mills, LN;Gray, JE
释放钙的第二信使肌醇1,4,5-三磷酸参与ABA对气孔孔径的调节。在其他信号通路中，肌醇1,4,5-三磷酸是由磷脂酶C的作用产生的。我们研究了磷脂酶C在保护细胞aba信号通路中的重要性。钙活化磷脂酶C的免疫定位证实了磷脂酶C在烟草保护细胞中的存在。保护细胞中磷脂酶C水平显著降低的转基因烟草植株在ABA作用下只能部分调节气孔开度。这些结果表明，磷脂酶C参与了ABA作用下气孔孔径减小的钙信号的扩增。由于没有观察到aba诱导的气孔开放的完全抑制，我们的研究结果支持其他钙释放第二信使在保护细胞aba信号通路中的作用。目前尚不清楚这些不同的钙释放第二信使是否在相同或平行的aba信号通路中起作用。
The calcium-releasing second messenger inositol 1,4,5-trisphosphate is involved in the regulation of stomatal aperture by ABA. In other signalling pathways, inositol 1,4,5-trisphosphate is generated by the action of phospholipase C. We have studied the importance of phospholipase C in guard cell ABA-signalling pathways. Immunolocalisation of a calcium-activated phospholipase C confirmed the presence of phospholipase C in tobacco guard cells. Transgenic tobacco plants with considerably reduced levels of phospholipase C in their guard cells were only partially able to regulate their stomatal apertures in response to ABA. These results suggest that phospholipase C is involved in the amplification of the calcium signal responsible for reductions in stomatal aperture in response to ABA. As full ABA-induced inhibition of stomatal opening was not observed, our results support a role for the action of other calcium-releasing second messengers in the guard cell ABA-signalling pathway. It is not known whether these different calcium-releasing second messengers act in the same or parallel ABA-signalling pathways.