HUMAN-LIVER PHOSPHATASE 2A - CDNA AND AMINO-ACID SEQUENCE OF 2 CATALYTIC SUBUNIT ISOTYPES
HUMAN-LIVER PHOSPHATASE 2A - CDNA AND AMINO-ACID SEQUENCE OF 2 CATALYTIC SUBUNIT ISOTYPES
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DOI：
10.1073/pnas.85.12.4252
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发表时间：
1988-06-01
影响因子：
11.1
通讯作者：
JOHNSON, GL
中科院分区：
文献类型：
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作者：
ARINO, J;CHEE, WW;JOHNSON, GL
从人肝脏文库中分离到两个编码两个磷酸酶2A催化亚基的cDNA克隆。这两种cdna在8个氨基酸上存在差异（97%同源性），并有3个非保守性替换。所有的氨基酸取代都聚集在蛋白质的氨基末端区域。一个人肝脏克隆（HL-14）的氨基酸序列与兔骨骼肌磷酸酶2A cDNA完全相同（核苷酸同源性为97%）。第二个人肝脏克隆（HL-1）由一个单独的基因编码，RNA凝胶印迹分析表明，这两个mrna在几种人克隆细胞系中表达相似。与磷酸酶1和2A的序列比较表明，在蛋白质的氨基端和羧基端氨基酸序列高度不同，并在两种蛋白质之间鉴定出六个高度保守的区域，这些区域预计对磷酸酶的酶活性很重要。
Two cDNA clones were isolated from a human liver library that encode two phosphatase 2A catalytic subunits. The two cDNAs differed in eight amino acids (97% identity) with three nonconservative substitutions,. All of the amino acid substitutions were clustered in the amino-terminal domain of the protein. Amino acid sequence of one human liver clone (HL-14)was identical to the rabbit skeletal muscle phosphatase 2A cDNA (with 97% nucleotide identity). The second human liver clone (HL-1) is encoded by a separate gene, and RNA gel blot analysis indicates that both mRNAs are expressed similarly in several human clonal cell lines. Sequence comparison with phosphatase 1 and 2A indicates highly divergent amino acid sequences at the amino and carboxyl termini of the proteins and identifies six highly conserved regions between the two proteins that are predicted to be important for phosphatase enzymatic activity.