EZ spheres: A stable and expandable culture system for the generation of pre-rosette multipotent stem cells from human ESCs and iPSCs
EZ spheres: A stable and expandable culture system for the generation of pre-rosette multipotent stem cells from human ESCs and iPSCs
复制标题
DOI：
10.1016/j.scr.2013.01.009
复制
发表时间：
2013-05-01
影响因子：
1.2
通讯作者：
Svendsen, Clive N.
中科院分区：
文献类型：
--
作者：
Ebert, Allison D.;Shelley, Brandon C.;Svendsen, Clive N.
我们已经开发了一种简单的方法来从人胚胎干细胞（hESC）和人诱导多能干细胞（iPSC）产生和扩增多能的、自我更新的玫瑰花结前神经干细胞，而不利用胚状体形成、手动选择技术或小分子的复杂组合。将人ESC和iPSC集落提起并置于含有高浓度EGF和FGF-2的神经干细胞培养基中。细胞聚集体（称为EZ球）可以使用保持细胞接触的切碎方法长时间扩增。早期传代EZ球迅速下调OCT 4和上调SOX 2和巢蛋白表达。它们保留了形成神经细胞的潜力，并始终分化成一系列中枢和外周神经谱系。因此，它们代表了一种非常早期的神经干细胞，比之前描述的其他方法具有更大的分化灵活性。因此，它们将用于快速扩展的神经发育和疾病建模，高内容筛选和基于多能干细胞技术的再生疗法领域。(C)2013爱思唯尔有限公司版权所有。
We have developed a simple method to generate and expand multipotent, self-renewing pre-rosette neural stem cells from both human embryonic stem cells (hESCs) and human induced pluripotent stem cells (iPSCs) without utilizing embryoid body formation, manual selection techniques, or complex combinations of small molecules. Human ESC and iPSC colonies were lifted and placed in a neural stem cell medium containing high concentrations of EGF and FGF-2. Cell aggregates (termed EZ spheres) could be expanded for long periods using a chopping method that maintained cell cell contact. Early passage EZ spheres rapidly down-regulated OCT4 and up-regulated SOX2 and nestin expression. They retained the potential to form neural rosettes and consistently differentiated into a range of central and peripheral neural lineages. Thus, they represent a very early neural stem cell with greater differentiation flexibility than other previously described methods. As such, they will be useful for the rapidly expanding field of neurological development and disease modeling, high-content screening, and regenerative therapies based on pluripotent stem cell technology. (C) 2013 Elsevier B.V. All rights reserved.