P53 IMMUNOREACTIVITY IN HUMAN-MALIGNANT MELANOMA AND DYSPLASTIC NEVI
P53 IMMUNOREACTIVITY IN HUMAN-MALIGNANT MELANOMA AND DYSPLASTIC NEVI
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DOI：
10.1111/j.1365-2133.1993.tb00253.x
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发表时间：
1993-06-01
影响因子：
10.3
通讯作者：
MACDONALD, DM
中科院分区：
文献类型：
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作者：
MCGREGOR, JM;YU, CCW;MACDONALD, DM
用抗重组人P53蛋白的抗血清CM-1进行免疫组织化学研究，检测了77例皮肤黑素细胞病变和5例恶性黑色素瘤淋巴结转移中肿瘤抑制蛋白P53的表达。由于野生型P53蛋白在正常细胞中迅速降解，p53免疫反应提示存在异常稳定的p53蛋白。这可能通过翻译后机制或基因突变发生。在黑素细胞病变中，P53免疫反应与恶性程度之间存在高度显著的相关性(P<；0.0001)。总体而言，63%的肿瘤标本中P53免疫反应阳性，但良性黑色素细胞痣中未见P53免疫反应，尽管少数发育不良的色素痣和1例孤立性Spitz痣中偶有核P53免疫反应弱的病灶。P53蛋白的表达与恶性黑色素瘤的预后密切相关(P=0.008)，提示P53抑癌蛋白在皮肤恶性黑色素瘤的生物学过程中起着重要作用。
Expression of the tumour suppressor protein, p53, was determined in 77 cutaneous melanocytic lesions, and in five lymph node metastases from malignant melanoma, in an immunohistochemical study employing CM-1, an antiserum raised against recombinant human p53 protein. Because wild-type p53 protein is rapidly degraded in normal cells, p 5 3 immunoreactivity suggests the presence of an abnormally stable p 5 3 protein. This may occur through either post-translational mechanisms or gene mutation.A highly significant correlation was found between p53 immunoreactivity and malignancy in melanocytic lesions (P < 0.0001). Overall, p53 immunoreactivity was observed in 63% of tumour specimens examined, but not in benign melanocytic naevi, although occasional foci of weak nuclear p53 immunoreactivity were observed in a minority of dysplastic naevi and a solitary Spitz naevus. A significant correlation was also found between strong p53 immunoreactivity and malignant melanomas associated with a poor prognosis (P = 0.008).These data suggest an important role for p 5 3 tumour suppressor protein in the biology of human cutaneous malignant melanoma.