The effect of the removal of sialic acid, galactose and total carbohydrate on the functional activity of Campath-1H
The effect of the removal of sialic acid, galactose and total carbohydrate on the functional activity of Campath-1H
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DOI：
10.1016/0161-5890(95)00118-2
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发表时间：
1995-12-01
影响因子：
3.6
通讯作者：
Patel, AK
中科院分区：
文献类型：
--
作者：
Boyd, PN;Lines, AC;Patel, AK
将单克隆人IgGl，Campath-IH，用糖苷酶消化以评估碳水化合物对IgGl的功能活性的影响。去除完整的碳水化合物部分消除了补体裂解活性和抗体依赖性细胞介导的细胞毒性，但保留了抗原结合活性和蛋白A结合活性。通过糖肽酶F消化去除末端唾液酸残基未发现影响任何测试的IgG活性。发现从去唾液酸化的Campath-1H去除大部分半乳糖残基降低但不消除补体裂解活性。其他活动不受去半乳糖基化的影响。这表明在受控条件下，IgG的补体裂解活性和抗体依赖性细胞介导的细胞毒性以其行为方式罕见分离。本文强调了糖在IgG功能中的整体重要性，并强调了一些糖残基的相对贡献。
A monoclonal human IgGl, Campath-lH, was digested with glycosidases to assess the effect of carbohydrate on the functional activities of an IgGl. Removal of the complete carbohydrate moiety abolished complement lysis activity and antibody-dependent cell-mediated cytotoxicity, but left antigen binding activity and protein A binding activity intact Removal of terminal sialic acid residues through glycopeptidase F digestion was not found to effect any of the tested IgG activities. Removal of the majority of the galactose residues from desialylated Campath-lH was found to reduce but not abolish complement lysis activity. Other activities were not affected by degalactosylation. This indicates a rare separation of complement lysis activity and antibody-dependent cell-mediated cytotoxicity of IgG in the way they behave under controlled conditions. This paper underlines the overall importance of carbohydrate in IgG function and stresses the relative contributions of some of the carbohydrate residues.