Electron spin resonance studies of changes in membrane fluidity of Chinese hamster ovary cells during the cell cycle.
Electron spin resonance studies of changes in membrane fluidity of Chinese hamster ovary cells during the cell cycle.
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DOI：
10.1016/0005-2736(80)90294-1
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发表时间：
1980-10
期刊：
影响因子：
--
通讯作者：
C. Lai;L. Hopwood;H. Swartz
中科院分区：
文献类型：
--
作者：
C. Lai;L. Hopwood;H. Swartz
以5-羟基硬脂酸为探针，采用电子自旋共振（ESR）自旋标记法研究了中国仓鼠卵巢（CHO）细胞周期中膜的流动性。建立了一种35 GHz ESR技术来研究完整细胞的膜流动性。与传统的自旋标记技术相比，这种技术只需要大约16个细胞。利用该技术，我们观察到CHO细胞在细胞周期中膜流动性的周期性变化：有丝分裂时细胞的膜流动性最高，而g1期和早期S期细胞的膜流动性最低。
Electron spin resonance (ESR) spin-label methods were used with 5-doxyl-stearic acid as a probe to investigate membrane fluidity of Chinese hamster ovary (CHO) cells during the cell cycle. A 35 GHz ESR technique was developed to study membrane fluidity of intact cells. This technique requires only about 1 6 the amount of cells compared to the conventional spin-label techniques. With this technique we observed a cyclic change of membrane fluidity during the cell cycle of CHO cells: cells in mitosis had the highest membrane fluidity, whereas cells in G 1 and early S phases had the lowest membrane fluidity.