The X boxes from promoters of HLA class II B genes at different loci do not complete for nuclear protein-specific binding.
The X boxes from promoters of HLA class II B genes at different loci do not complete for nuclear protein-specific binding.
复制标题
来自不同位点的 HLA II B 类基因启动子的 X 盒不完成核蛋白特异性结合。
DOI：
10.1007/bf00210449
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发表时间：
1990
期刊：
影响因子：
3.2
通讯作者：
Trucco,M
中科院分区：
文献类型：
--
作者：
Turco,E;Manfras,BJ;Ge,L;Rudert,WA;Trucco,M
HLA Ⅱ类基因的表达受转录起始位点5'端上游启动子区的顺式作用元件的协同调控，细胞核蛋白中的反式作用因子通过与高度保守的序列（称为盒）结合，对这些基因的转录进行正或负调控。在克隆细胞系Priess中存在的所有转录的IIB类基因的启动子区域后，我们能够识别某些蛋白盒复合物，并通过比较每个基因的启动子盒来确定这些蛋白对其各自盒的亲和力。其他基因。不同的核蛋白似乎与所测试的不同IIB类基因的X盒结合。在Y盒-蛋白质复合物的情况下，各种Y盒以相似的亲和力竞争。与DRB 1-CCAAT盒特异性结合的蛋白质也与DPB 1-CCAAT盒结合，但完全不能与同源盒DQB 1结合。此外，CCAAT盒特异性蛋白不结合相同基因的Y盒，排除了这些蛋白仅识别Y中存在的反向CCAAT盒（ATTGG）的可能性。
Expression ofHLAclass II genes is coordinately regulated bycis-acting elements present in their promoter regions immediately upstream from the 5' end of their tranccription start sites.Trans-acting factors from the nuclear proteins of the cell are able to positively or negatively regulate transcription of these genes by binding to highly conserved sequences, called boxes. After cloning the promoter regions of all the transcribed class IIBgenes present in the cell line Priess, we were able to identify certain protein-box complexes and to determine the affinity of these proteins for their respective boxes by comparing promoter boxes of each gene to those of the other genes. Different nuclear proteins seemed to bind to the X boxes of the different class IIBgenes tested. In the case of the Y box-protein complexes, the various Y boxes competed with similar affinities. The protein(s) which specifically bound to the DRB1-CCAAT box also bound to DPB1-CCAAT box, but completely failed to bind the homologous box formDQB1. Further, CCAAT box-specific protein(s) did not bind to the Y box of the same gene, excluding the possibility that these proteins just recognize the reverse CCAAT box (ATTGG) present within Y.