Physiological properties of pain-modulating neurons in rostral ventromedial medulla in female rats, and responses to opioid administration.
Physiological properties of pain-modulating neurons in rostral ventromedial medulla in female rats, and responses to opioid administration.
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DOI：
10.1016/j.ynpai.2021.100075
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发表时间：
2021-08
期刊：
影响因子：
--
通讯作者：
Heinricher MM
中科院分区：
文献类型：
--
作者：
Hryciw G;De Preter CC;Wong J;Heinricher MM
描述了雌性大鼠RVM疼痛调节神经元的生理特性。女性的ON和OFF细胞具有与男性相当的基本特性。在男性中，RVM神经元输出在持续性炎症和吗啡中改变。本研究为进一步研究女性RVM奠定了基础。功能性疼痛障碍不成比例地影响女性，但大多数动物疼痛研究都是在男性中进行的。虽然脑干疼痛调制回路中存在解剖学和药理学上的性二态性，但尚未在雌性动物中探索包括主要功能输出（延髓头端腹内侧区（RVM））的疼痛调制神经元的生理学。本研究的目的是鉴定和表征雌性大鼠与雄性大鼠相比RVM细胞的活性。在雌性动物的RVM内识别出ON和OFF细胞，其放电特性与雄性动物中描述的放电特性相当。此外，ON-和OFF-细胞表现出敏化反应的躯体刺激在女性进行持续性炎症，和ON-和OFF-细胞响应全身给药吗啡的剂量足以产生行为抗伤害感受。这些数据表明，ON-/OFF-细胞框架最初定义在男性也存在于女性，并在男性，这些神经元在女性中招募持续炎症和全身给予吗啡。重要的是，这项工作为在RVM和下降控制研究中使用雌性动物奠定了基础。
Physiological properties of RVM pain-modulating neurons were described in female rats. ON- and OFF-cells in females have fundamental properties comparable to those in males. As in males, RVM neuron output is altered in persistent inflammation and by morphine. This work provides a foundation for future studies of RVM in females. Functional pain disorders disproportionately impact females, but most pain research in animals has been conducted in males. While there are anatomical and pharmacological sexual dimorphisms in brainstem pain-modulation circuits, the physiology of pain-modulating neurons that comprise a major functional output, the rostral ventromedial medulla (RVM), has not been explored in female animals. The goal of this study was to identify and characterize the activity of RVM cells in female, compared to male, rats. ON- and OFF-cells were identified within the RVM in females, with firing properties comparable to those described in males. In addition, both ON- and OFF-cells exhibited a sensitized response to somatic stimuli in females subjected to persistent inflammation, and both ON- and OFF-cells responded to systemically administered morphine at a dose sufficient to produce behavioral antinociception. These data demonstrate that the ON-/OFF-cell framework originally defined in males is also present in females, and that as in males, these neurons are recruited in females in persistent inflammation and by systemically administered morphine. Importantly, this work establishes a foundation for the use of female animals in studies of RVM and descending control.