Marinifaba aquimaris gen. nov., sp. nov., a novel chitin-degrading gammaproteobacterium in the family Alteromonadaceae isolated from seawater of the Mariana Trench
Marinifaba aquimaris gen. nov., sp. nov., a novel chitin-degrading gammaproteobacterium in the family Alteromonadaceae isolated from seawater of the Mariana Trench
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马里尼法巴 aquimaris gen.
DOI：
10.1007/s10482-021-01568-w
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发表时间：
2021
影响因子：
2.6
通讯作者：
Zhang Xi-ying
中科院分区：
文献类型：
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作者：
Sui Xin;He Xiao-yan;Liu Ning-hua;Dang Yan-ru;Cha Qian-qian;Sun Mei-ling;Li Chun-yang;Fu Hui-hui;Song Xiao-yan;Qin Qi-long;Chen Xiu-lan;Zhang Yu-zhong;Su Hai-nan;Zhang Xi-ying
从马里亚纳海沟采集的海水样品中分离到一株革兰氏阴性、杆状、好氧、氧化酶阳性和过氧化氢酶阴性的细菌，命名为SM 1970 T。菌株SM 1970 T在1 - 5 - 37 ℃和1-5%（w/v）NaCl中生长。它水解胶体甲壳素，琼脂和酪蛋白，但不还原硝酸盐为亚硝酸盐。基于16 S rRNA基因序列的系统发育分析表明，菌株SM 1970 T形成了一个独特的谱系，接近交替单胞菌科Catenovulum属，共享最高的序列相似性与海洋链霉菌模式株SM 1970 T的同源性为93.6%，与γ-变形菌纲其他已知种的同源性< 93.0%，其主要脂肪酸组成为特征3（C16： 1ω 7 c和/或C16： 1ω 6 c）、C16： 0和求和特征8（C18： 1ω 7 c和/或C18： 1ω 6 c）。该菌的主要极性脂为磷脂酰乙醇胺和磷脂酰甘油，呼吸醌主要为泛醌8。SM 1970 T菌株基因组草图由77个支架组成，全长4，172，146 bp，包含一套完整的几丁质降解基因。SM 1970 T与已知链霉菌模式菌株的氨基酸序列平均同源性（AAI）为56.6-57.1%，保守蛋白质百分比（POCP）为28.5- 31.5%。菌株SM 1970 T基因组DNA G + C含量为40.1mol%。根据多相分析，菌株SM 1970 T代表交替单胞菌科一新属中的一新种，并建议将其命名为Marinifaba aquimentum，模式菌株为SM 1970 T（= MCCC 1 K 04323 T = KCTC 72844 T）。
A novel Gram-negative, rod-shaped, aerobic, oxidase-positive and catalase-negative bacterium, designated strain SM1970T, was isolated from a seawater sample collected from the Mariana Trench. Strain SM1970Tgrew at 15-37oC and with 1–5% (w/v) NaCl. It hydrolyzed colloidal chitin, agar and casein but did not reduce nitrate to nitrite. Phylogenetic analysis based on the 16S rRNA gene sequences revealed that strain SM1970Tformed a distinct lineage close to the genusCatenovulumwithin the familyAlteromonadaceae, sharing the highest sequence similarity (93.6%) with type strain ofCatenovulum maritimumbut < 93.0% sequence similarity with those of other known species in the classGammaproteobacteria.The major fatty acids of strain SM1970Twere summed feature 3 (C16: 1ω7cand/or C16: 1ω6c), C16: 0and summed feature 8 (C18: 1ω7cand/or C18: 1ω6c). The major polar lipids of the strain included phosphatidylethanolamine and phosphatidylglycerol and its main respiratory quinone was ubiquinone 8. The draft genome of strain SM1970Tconsisted of 77 scaffolds and was 4,172,146 bp in length, containing a complete set of genes for chitin degradation. The average amino acid identity (AAI) values between SM1970Tand type strains of knownCatenovulumspecies were 56.6–57.1% while the percentage of conserved proteins (POCP) values between them were 28.5–31.5%. The genomic DNA G + C content of strain SM1970Twas 40.1 mol%. On the basis of the polyphasic analysis, strain SM1970Tis considered to represent a novel species in a novel genus of the familyAlteromonadaceae, for which the nameMarinifaba aquimarisis proposed with the type strain being SM1970T(= MCCC 1K04323T= KCTC 72844T).